Detecting the occurrence of indigenous and non-indigenous megafauna through fishermen knowledge: A complementary tool to coastal and port surveys.

Marine bioinvasions and other rapid biodiversity changes require today integrating existing monitoring tools with other complementary detection strategies to provide a more efficient management. Here we explored the efficacy of fishermen observations and traditional port surveys to effectively track the occurrence of both indigenous and non-indigenous megafauna in the Adriatic Sea. This consisted mainly of mobile taxa such as fishes, crustaceans and molluscs. Port surveys using traps and nets within 10 major Adriatic harbours, were compared with the information obtained from 153 interviews with local fishermen. Information gathered by traps and nets varied significantly and generally resulted of limited efficacy in exotic species detection. Interviews allowed tracking the occurrence of new species through time and space, providing complementary knowledge at the low cost. This combined approach improves our capability of being informed on the arrival of species of different origin, providing a more rational, improved basis for environmental management and decision making.

Floating plastics in Adriatic waters (Mediterranean Sea): From the macro- to the micro-scale.

Macro- and microplastics abundances were determined in the Adriatic Sea following the MSFD TG10 protocol. The studied areas included populated gulfs, river outlets and offshore waters in five Adriatic countries. The use of small ships enabled us to detect small sized plastics (2.5-5 cm) and record average macroplastics densities of 251 ±â€¯601 items km-2, one order of magnitude higher than previously considered. Results from manta net tows for microplastics revealed an average abundance of 315,009 ±â€¯568,578 items km-2 (217 ±â€¯575 g km-2). We found significantly higher microplastics abundances in nearshore (≤4 km) than in offshore waters (>4 km) and this trend seems to affect also the small sized macro plastic fragments (2.5-5 cm). The dominant polymers were polyethylene and polypropylene while the presence of some rare polymers and waxes used in food and dentistry indicated waste water treatment plants as potential sources of microplastics.

Floating marine litter as a raft for drifting voyages for Planes minutus (Crustacea: Decapoda: Grapsidae) and Liocarcinus navigator (Crustacea: Decapoda: Polybiidae).

The Columbus crab Planes minutus and Arch-fronted swimming crab Liocarcinus navigator, within their distribution ranges in the Mediterranean, were found rafted on plastic macro-litter floating on the open south Adriatic. While P. minutus was recorded from inanimate flotsam outside of the Mediterranean, L. navigator is herein reported for the first time on floating marine litter. The role of floating litter as habitat or as a dispersal agent for marine invertebrates has received quite attention however, records of decapod crabs drifting on litter has been relatively sparse. Our results suggests that vast quantities of floating debris, comprised primarily of non-biodegradable plastic polymers, probably will augment natural floating substrates in the marine environment, potentially facilitating the spread of invasive species. The dispersion of rafting crabs through floating debris should be investigated given the high potential ecological risk of invasion by exotic species due to the increase in waste production (ecological risk assessment).

The spawning, embryonic and early larval development of the green wrasse Labrus viridis (Linnaeus, 1758) (Labridae) in controlled conditions.

Green wrasse, Labrus viridis (Linnaeus, 1758), is an endangered species in the southern Adriatic Sea, but it is also of interest for potential rearing in polyculture with other commercial species for the repopulation of areas where it is endangered or as a new aquaculture species. A parental stock of the green wrasse was kept in aquaria for six years. The spawning, embryonic and early larval development maintained under controlled laboratory conditions are described and illustrated. The average diameter of newly spawned eggs was 1.01±0.03 mm. Mature and fertilized eggs were attached to the tank bottom by mucus. Hatching started after 127 h at a mean temperature of 14.4±0.8°C. The average total length of newly hatched larvae was 4.80±0.22 mm. Absorption of the yolk-sac was completed after the 5th day when larvae reached 5.87±0.28 mm. Larvae were fed with the rotifers Brachionus plicatilis. The pigmentation of L. viridis larvae is similar to that of Labrus merula and Labrus bergylta, but the main differences between these species are in the size of larvae and the development time of the melanophores on the anal fin-fold (five days later than with L. merula) and on top of the head (nine days earlier than with L. merula).