[Experimental study of the biopreparation Lymphotilin as an antiproliferative and antitumor agent]. / Eksperimental'noe izucheniie biopreparata Limfotilin kak antiproliferativnogo i protivoopukholevogo sredstva.

We found in experiments involving the use of a biopreparation lymphotilin that its administration was followed by a decrease in proliferative activity of cultured tumor cells and a longer survival of mice bearing transplantable leukemia. An intensified intercalation of ethidium bromide in nucleic acids of tumor cells in lymphotilin culture points to the drug activity on nuclear level. Tumor cell inhibition by lymphotilin holds much promise for the practice of hematology.

[Leukocyte and thrombocyte glycosaminoglycans in hemophilia A and von Willebrand's disease]. / Glikozaminoglikany leikotsitov i trombotsitov pri gemofilii A i bolezni Villebranda.

AIM: The study of glycosaminoglycanes (GAG) in leukocytes and platelets of patients with hereditary coagulopathy. MATERIALS AND METHODS: GAG concentration, composition and fraction identification were made in 25 patients with hemophilia A and 10 patients with Willebrand disease. RESULTS: In hemophiliacs, leukocytes contained low concentrations of GAG. In those with bleeding and synovitis GAG levels were lower than the average, in those with extensive hematomas in the absence of locomotor disorders the above levels were close to normal. Chondroitinsulphate dominated in GAG composition though it was less polydisperse. Heparin sulphate levels were elevated. Platelet GAG characteristics were close to normal. In Willebrand disease leukocyte GAG content and composition was similar to those in hemophilia A except some differences in electrophoretic properties of small GAG components. CONCLUSION: Metabolism and/or release of GAG from blood cells may be involved in pathogenesis of hemophilia A and Willebrand disease.

[Effects of a lymph node extract on hemopoietic and lymphoid cells in normal state and in different forms of leukemia]. / Vliianie ékstrakta limfaticheskikh uzlov na gemopoéticheskie i limfoidnye kletki v norme i pri razlichnykh formakh leikoza.

The chemical composition and properties of the lymphatic node extract (LNE) obtained from the porcine submandibular lymphatic nodes have been studied. It has been established that the LNE is the mixture of low-molecular weight peptides and the components of nucleic acids with the molecular mass below 2 kD. It possesses antiproliferative, immunosuppressive and antileukemic activities in vitro and in vivo. The inhibitory effect was the most pronounced in respect to lymphoid cells rather than myeloid cells of the patients with leukemia.