Analysis of the impacts of horizontal translation and scaling on wavefront approximation coefficients with rectangular pupils for Chebyshev and Legendre polynomials.

Chebyshev and Legendre polynomials are frequently used in rectangular pupils for wavefront approximation. Ideally, the dataset completely fits with the polynomial basis, which provides the full-pupil approximation coefficients and the corresponding geometric aberrations. However, if there are horizontal translation and scaling, the terms in the original polynomials will become the linear combinations of the coefficients of the other terms. This paper introduces analytical expressions for two typical situations after translation and scaling. With a small translation, first-order Taylor expansion could be used to simplify the computation. Several representative terms could be selected as inputs to compute the coefficient changes before and after translation and scaling. Results show that the outcomes of the analytical solutions and the approximated values under discrete sampling are consistent. With the computation of a group of randomly generated coefficients, we contrasted the changes under different translation and scaling conditions. The larger ratios correlate the larger deviation from the approximated values to the original ones. Finally, we analyzed the peak-to-valley (PV) and root mean square (RMS) deviations from the uses of the first-order approximation and the direct expansion under different translation values. The results show that when the translation is less than 4%, the most deviated 5th term in the first-order 1D-Legendre expansion has a PV deviation less than 7% and an RMS deviation less than 2%. The analytical expressions and the computed results under discrete sampling given in this paper for the multiple typical function basis during translation and scaling in the rectangular areas could be applied in wavefront approximation and analysis.

Carrier squeezing interferometry: suppressing phase errors from the inaccurate phase shift.

The carrier squeezing interferometry algorithm is proposed to retrieve the phase from interferograms with phase shift errors. A linear carrier is introduced in the interferograms, and the image data is rearranged by the squeezing interferometry technology. In the spectrum of the rearranged image, the error lobe and the phase lobe are separated so the error-free phase can be retrieved by filtering. The simulated interferograms with phase shift errors are computed, and the precisions are better than 8.4×10(-4)λ. Its validation is verified by experiments, where a mean precision of 0.0040λ is obtained.

The systemic evaluation and clinical significance of immunological function for advanced lung cancer patients / 中国肺癌杂志

<p><b>BACKGROUND AND OBJECTIVE</b>The actual evaluation of immunological function is significant for studing the tumor development and devising a treatment in time. The aim of this study is to evaluate the immunological function of advanced lung cancer patients systematically, and to discuss the clinical significance.</p><p><b>METHODS</b>The nucleated cell amounts of advanced lung cancer patients and the healthy individuals were counted. The immune cell subsets and the levels of IL-4, INF-gamma, perforin and granzyme in CD8+T cells by the flow cytometry were measured. The proliferation activity and the inhibition ratio of immune cells to several tumor cell lines were evaluated by MTT assay.</p><p><b>RESULTS</b>The absolute amounts and subsets of T, B, NK cells of advanced lung cancer patients were lower than the healthy individuals (P < 0.05); However, the proportion of regulatory T cells of advanced lung cancer patients (4.00 +/- 1.84)% was lower than the healthy individuals (1.27 +/- 0.78)% (P < 0.05). The positive rates of IFN-gamma perforin, granzyme in CD8+T cells decreased while them in IL-4 did not in the advanced lung cancer patients compared to the healthy control group (P < 0.05). The proliferation activity of immune cells, the positive rate of PPD masculine and the inhibition ratio to tumor cells in the advanced lung cancer patients was lower than the healthy subsets obviously (P < 0.05).</p><p><b>CONCLUSION</b>There was a significant immune depression in the advanced lung cancer patients compared to the healthy individuals.</p>

Biological characteristics and antitumor activity of CIK cells activated by recombinant human fibronectin for human lung cancer cell lines in vitro / 中国肺癌杂志

<p><b>BACKGROUND AND OBJECTIVE</b>The CIK cell is one of the most important means of the adoptive cellular immunotherapy, and it is a hotspot of which to simplify its culture procedure and to promote its inhibition rate. The aim of this study is to observe the biological function of the CIK cells cultivated by the recombinant human fibronectin (RN) and to establish an effective and simple way of cells expansion.</p><p><b>METHODS</b>We separated the mononuclear cells (PBMCs) in 50 mL peripheral blood from 10 healthy persons with density gradient centrifugation in the lymphocyte-separating medium, and the PBMCs were divided into two groups, of which were cultivated by RN-introduced and conventional method separately. Then we estimated the proliferation ability, and analyzed the immunologic type, IFN-gamma, IL-4, perforin and granzyme B of them with flow cytometry. Besides that, we tested the inhibition rate of CIKs cells to four kinds of human lung cancer cell lines in vitro by MTT assay.</p><p><b>RESULTS</b>The RN-induced group had a higher proliferation rate that was 2.0-3.5 times of the conventional group, and there was an obvious statistical difference between the two (P < 0.05). The proliferation rates of CD3+CD16+CD56+T cells in each group were 3 778 and 2 068 times of the initial number, respectively. There was also a higher percentage of CD3+CD8+ T cells in RN-induced group (P < 0.05), while the percentage of CD3+CD4+T cells had no significant statistical difference (P > 0.05). We found a similar inhibition rate of the CIK cells to all this human lung cancer cell lines (P > 0.05). The cells which secreted IFN-gamma increased, while the cells which secreted IL-4 did not. The cells which secreted granzyme B and perforin were positive.</p><p><b>CONCLUSION</b>It is an effective and simple way to cultivate the CIK cells with RN, which should be adopted.</p>