RESUMO
In experiment on animals, in which the cold trauma was simulated, the systemic inflammatory reactions indiceslevel of Сreactive protein, аctive products of thiobarbituric acid the blood serum, erythrocyte sedimentation rate (ESR), leukocyte blood formula (LBF) while injection of placenta extract were studied. Creactive protein level lowering in 1.3 times on the 14th day of experiment, reduction of ESR in 1.6 times on the 7th and 14th day, LBF normalization on the 7th day, lowering of the аctive products of thiobarbituric acid level in 1.3 times on the 7, 9th and 14th day of observation were noted. On the 21st day all the indices came back to norm.
Assuntos
Lesão por Frio/prevenção & controle , Misturas Complexas/farmacologia , Placenta/química , Substâncias Protetoras/farmacologia , Regeneração/efeitos dos fármacos , Animais , Sedimentação Sanguínea , Proteína C-Reativa/metabolismo , Lesão por Frio/sangue , Lesão por Frio/patologia , Misturas Complexas/isolamento & purificação , Feminino , Injeções Intramusculares , Contagem de Leucócitos , Gravidez , Substâncias Protetoras/isolamento & purificação , Ratos , Regeneração/fisiologia , Pele/lesões , Suínos , Tiobarbitúricos/sangueRESUMO
The paper is devoted to the investigation of the lipid peroxidation level according to measurements of the accumulation level of malon dialdehyde in fragments of the liver tissue of newborn and adult pigs, depending on the cooling rate and cryoprotectants used. The conclusion has been made on the basis of the data obtained. That the lipid peroxidation level in the liver tissue fragments is decreased with the increase of cooling rates. That is activated the least of all with the use of average cooling rate (100 degrees C/min) with dimethylsulphoxide (DMSO) as cryoprotectant in concentrations of 10, 20% and rapid cooling rate (80,000 degrees C/min) with polyethylenglycol-1500 (PEG-1500) and DMSO in analogous concentrations. These cryopreservation data have shown the lowest of the lipid peroxidation level in the liver tissue fragments when comparing one with native tissue (before freezing).