RESUMO
Translation initiation region (TIR) of the rpsA mRNA encoding ribosomal protein S1 is one of the most efficient in Escherichia coli despite the absence of a canonical Shine-Dalgarno-element. Its high efficiency is under strong negative autogenous control, a puzzling phenomenon as S1 has no strict sequence specificity. To define sequence and structural elements responsible for translational efficiency and autoregulation of the rpsA mRNA, a series of rpsA'-'lacZ chromosomal fusions bearing various mutations in the rpsA TIR was created and tested for beta-galactosidase activity in the absence and presence of excess S1. These in vivo results, as well as data obtained by in vitro techniques and phylogenetic comparison, allow us to propose a model for the structural and functional organization of the rpsA TIR specific for proteobacteria related to E.coli. According to the model, the high efficiency of translation initiation is provided by a specific fold of the rpsA leader forming a non-contiguous ribosome entry site, which is destroyed upon binding of free S1 when it acts as an autogenous repressor.
Assuntos
Proteínas de Bactérias/biossíntese , Conformação de Ácido Nucleico , Biossíntese de Proteínas , RNA Bacteriano/química , Proteínas Ribossômicas/biossíntese , Regiões 5' não Traduzidas , Proteínas de Bactérias/genética , Sequência de Bases , Sítios de Ligação , Escherichia coli/genética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Iniciação Traducional da Cadeia Peptídica , Filogenia , Proteobactérias/genética , Proteínas Ribossômicas/genéticaRESUMO
In an attempt to understand how Escherichia coli ribosomes recognize the initiator codon on mRNAs lacking the Shine-Dalgarno (SD) sequence, we have studied 30S initiation complex formation in extension inhibition (toeprinting) experiments using (-SD)mRNAs which are known to be reliably translated in E. coli: the plant viral messenger A1MV RNA 4 and two chimaeric mRNAs coding for beta-glucuronidase (GUS) and bearing the 5'-untranslated sequence of TMV RNA (omega) or the omega-derived sequence (CAA)n as 5'-leaders. Ribosomal protein S1 and IF3 have been found to be indispensable for translational initiation. Protein S1 appears to be a key recognition element. S1 binds to sequences within the leaders of (-SD)mRNAs thus providing their affinity to E. coli ribosomes.
Assuntos
Escherichia coli/metabolismo , RNA Mensageiro/metabolismo , Proteínas Ribossômicas/metabolismo , Ribossomos/metabolismo , Vírus do Mosaico da Alfafa/genética , Sequência de Bases , Códon/metabolismo , Primers do DNA , Glucuronidase/biossíntese , Dados de Sequência Molecular , Biossíntese de Proteínas , RNA Mensageiro/biossíntese , RNA Viral/metabolismo , Vírus do Mosaico do Tabaco/genéticaRESUMO
Ribosomal protein S1 is known to play an important role in translational initiation, being directly involved in recognition and binding of mRNAs by 30S ribosomal particles. Using a specially developed procedure based on efficient crosslinking of S1 to mRNA induced by UV irradiation, we have identified S1 binding sites on several phage RNAs in preinitiation complexes. Targets for S1 on Q beta and fr RNAs are localized upstream from the coat protein gene and contain oligo(U)-sequences. In the case of Q beta RNA, this S1 binding site overlaps the S-site for Q beta replicase and the site for S1 binding within a binary complex. It is reasonable that similar U-rich sequences represent S1 binding sites on bacterial mRNAs. To test this idea we have used E. coli ssb mRNA prepared in vitro with the T7 promoter/RNA polymerase system. By the methods of toeprinting, enzymatic footprinting, and UV crosslinking we have shown that binding of the ssb mRNA to 30S ribosomes is S1-dependent. The oligo(U)-sequence preceding the SD domain was found to be the target for S1. We propose that S1 binding sites, represented by pyrimidine-rich sequences upstream from the SD region, serve as determinants involved in recognition of mRNA by the ribosome.