RESUMO
Two Pb(II)-resistant bacteria isolated from a soil containing 2,500 mg/kg of Pb were identified by 16S rRNA sequencing analysis as Delftia sp. and designated as 3C and 6C. Both isolates grew at a Pb(II) concentration of 62 mg/L and at the stationary phase showed a Pb(II)-sorption capability of 10 ± 1.5 (3C) and 5 ± 0.8 (6C) mg/g of biomass. Biochemical properties related to heavy metal resistance and plant growth promotion were analyzed and compared with the Cr(VI)-resistant plant growth-promoting Delftia sp. JD2, previously reported by our group. Both isolates and JD2 were resistant to Cr(VI), Pb(II) and many antibiotics, produced siderophores and the phytohormone indole-3-acetic, and showed clover growth-promoting activity in greenhouse conditions. Interestingly, the occurrence of integron class 1 was shown in all isolates. Our results add to previous reports and suggest that bacteria of the genus Delftia could be consider as good candidates for the design of technologies for cleaning up contaminated environments and/or the production of biofertilizers.
Assuntos
Biodegradação Ambiental , Delftia/isolamento & purificação , Delftia/fisiologia , Fertilizantes , Microbiologia do Solo , Antibacterianos/farmacologia , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Delftia/classificação , Delftia/genética , Farmacorresistência Bacteriana , Ácidos Indolacéticos/metabolismo , Chumbo/toxicidade , Dados de Sequência Molecular , Desenvolvimento Vegetal , Reguladores de Crescimento de Plantas/metabolismo , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
A chromium (Cr)-resistant bacterium isolated from soil containing 6,000 mg/kg of Cr was identified based on 16S rRNA gene sequence analysis as Delftia, and designated as JD2. Growth of JD2 was accompanied with reduction of Cr(VI) to Cr(III) in liquid medium initially containing 100 mg/L Cr(VI), the maximum concentration allowing growth. JD2 showed NADH/NADPH-dependent reductase activity associated with the soluble fraction of cells. The results suggest that JD2 might be a good candidate for the treatment of highly Cr(VI)-contaminated water and/or industrial effluents. The isolate produced indole-3-acetic acid in the presence and absence of Cr(VI) and showed free-living nitrogen-fixing activity possibly attributable to a V-nitrogenase. JD2 did not counteract the harmful effect of Cr(VI) during leguminous plant growth and nodulation by rhizobial strains but functioned as a "helper" bacterium to enhance the performance of rhizobial inoculant strains during inoculation of alfalfa and clover (used as model plants to study plant growth-promoting activity) in the absence of Cr(VI).
Assuntos
Cromo/metabolismo , Delftia/fisiologia , Medicago sativa/microbiologia , Microbiologia do Solo , Trifolium/microbiologia , Biodegradação Ambiental , Cromo/toxicidade , Delftia/efeitos dos fármacos , Delftia/enzimologia , Delftia/genética , Delftia/crescimento & desenvolvimento , Farmacorresistência Bacteriana/fisiologia , Ácidos Indolacéticos/metabolismo , Medicago sativa/crescimento & desenvolvimento , Dados de Sequência Molecular , Fixação de Nitrogênio/fisiologia , Oxirredução , Oxirredutases/metabolismo , RNA Ribossômico 16S/genética , Sideróforos/metabolismo , Poluentes do Solo/toxicidade , Trifolium/genéticaRESUMO
Chromium (Cr)-resistant bacteria isolated from a soil with 6 g kg(-1) of Cr were identified based on 16S rRNA gene sequence analysis as a Stenotrophomonas, and designated as JD1. Growth of JD1 was accompanied by transformation of Cr(VI) to Cr(III) in liquid medium initially containing 300 mg L(-1) Cr(VI), the maximum concentration allowing growth. JD1 produced the highest levels of a Cr(VI)-binding exopolysaccharide when grown in medium with 100 mg L(-1) Cr(VI). The relative exopolysaccharide monosaccharide composition was analysed by HPLC, which showed that rhamnose+galactose was the major component, and that its relative level increased when cells were grown with Cr(VI). JD1 grew as a biofilm on various inert surfaces. Biofilm macromolecular composition analysis indicated that the relative levels of exopolysaccharide and protein were more abundant in biofilms grown in 100 mg L(-1) Cr(VI), whereas relative uronic acid levels remained constant. Biofilm cells exposed to Cr(VI) were elongated, grouped in clusters and exopolysaccharide obtained from the biofilm extracellular matrix had an enhanced capacity to bind Cr(VI). Exopolysaccharide production and composition, and biofilm growth are discussed as a mechanism of protection that allows survival during Cr(VI) stress.
