[Treatment of chronic viral hepatitis C with direct acting antiviral agent: review].

HCV infection treatment regimens are viewed from positions of HCV life cycle and replication, effects of NS3/4A protease inhibitors and NS5A/NS5B inhibitors on HCV strain replication. Evolution of HCV treatment regimens from its discovery to modern DAA agents had led to substantial progress although drug resistance poses a new issue to be addressed.

DETOXIFICATION AND DESENSITIZATION OF THE BODY IN THE TRADITIONAL MEDICINE / Монголын эм зүй, эм судлал

The present work presents information on the development and use of technologies for detoxification and desensitization of the body based on the tenets of the traditional medicine.KEY WORDS: traditional medicine, purification of the body, development and use, technology for detoxification and desensitization.INTRODUCTIONIn recent decades it has been noted the decrease in the index health as well as the incidence rate growth due to the deterioration of environmental conditions, the increased fastness of living, and the reduction of social protectability [7]. Pollution of the inner medium of the body with substances received through water, food and air causes a serious worry. These substances include plant and animal growth stimulants, fertilizers, pesticides, various coloring matters for foodstuffs, preservatives, drugs, salts of heavy metals, radionuclides, etc. Intensive xenobiotic contamination gives rise to dangerous acute intoxications and the development of chronic intoxication of the body followed by the disturbances of inner organs functioning resulting in the decrease of the bodily resistance and growth of incidence rate [2, 3]. Growing environmental and social pressing leads to the impairment of detoxification, desensitization, immune and other adaptive mechanisms of the human body [4, 5] that is a cause of many diseases. According to the data of World Health Organization (WHO) there is a tendency to the development of severe chronic cases due to intoxication and sensitization of the body [7]. Under the circumstances when the rate of chronic cases increases in number and the future generations are threatened with chronic diseases, the detoxification of the body seems of great importance.THE AIM of the given work is to develop and introduce into practice the detoxification and desensitization technology based on the traditional medicine.THE OBJECTS for studies were plant remedies stimulating detoxification and drainage function of the hepatobiliary system, gastro- intestinal tract, kidneys and skin created on the base of the traditional medicine formulae (nor bu dun thang, shi rguid, nephrophyt, sudorific plant collection). The studies were carried out with the use of pharmacological, clinical, biochemical and statistical methods [1, 6].RESULT. The lymphatic system is known to be the main drainage system excreting endo- and exotoxins from extracellular space, from cells and tissues [2, 3]. The system is capable to transport even big structures (high-molecular proteins, toxins) which enter the system through valves but not through semi-impermeable vessel walls as in the case of circulatory system. As a result transported toxins enter the venous duct. Further, the processes of detoxification take place mainly in the liver and kidneys. To avoid the “overloading” of these organs it is necessary to increase their functional activity using relevant medicinal preparations [4, 5, 7]. The increase in functioning of the given organs and drainage are regarded as the first level of detoxification and desensitization of the body. At present, taking into account the chemization of the home, water, air and food pollution and uncontrolled intake of medicinal preparations, it is necessary to pay attention to the “medicine of evacuation” i.e. to the purification of inner medium of the body [1, 5]. The technology for detoxification and desensitization of the body developed on the base of the traditional medicine, suggests the stage-by-stage intake of the remedy stimulating hepatobiliary system (nor bu dun thang) for 9 days; the remedy promoting the function of gastro-intestinal tract (shi brguid) for 9 days; the remedy stimulating the function of kidneys(nephrophyt) for 9 days and sudorific remedy (sudorific plant collection) for 9 days. Pre-clinical studies which were carried out on white male Wistar rats have revealed drainage and detoxification influence of the given remedies. The combined administration of the above remedies to SBA mice has shown that they have desensitization effect. Clinical studies of the given technology were carried out on the patients with endogenic intoxication and pollution of the inner medium of the body, particularly, on the persons suffered from atopic dermatitis and eczema. The Table 1 shows the results of therapy with the use of the developed technology of detoxification and desensitization of the body. The clinical studies were carried out on the patients suffered from allergic dermatoses (atopic dermatitis, eczema) at the Buryat Republican dermato-venerologic dispensary.The data given in the Table 1 show that the use of the given technology of detoxification and desensitization of the body improves the general state of the majority of patients followed by normalization of biochemical indices in the blood serum. Biochemical investigations performed after treatment have shown the decrease of the malonic dialdehyde (MDA) content and circulating immune complexes (CIC); the transaminase activity and concentration of α and α2 –globulins were also decreased as compared with the data obtained before treatment. The same tendency was observed in patients with the subacute stage of atopic dermatitis. Only in 5 patients no significant effect was noted. When the given technology was used for the treatment of eczema no significant positive changes in biochemical indices of the blood serum were noted in 32 cases in spite of satisfactory general state of the patients on the background of the treatment.The studies conducted have revealed the tendency to the improvement of the general state of the patients and the improvement of biochemical indices in the blood serum at the final terms of the treatment with the use of the given technology. Nevertheless in some cases a full health restoration was not noted in a number of patients, especially in patients with eczema. It is indicative of probable recurrence of the disease. Refresher course of the treatment is advisable.

[Effect of Hypecoum erectum extract on morphofunctional state of the liver in rats with tetracycline-associated hepatitis].

The effect of Hypecoum erectum L. extract on the morphofunctional condition of the liver in rats with experimental tetracycline-associated hepatitis was studied. The experiment included 40 albino rats Wistar. The animals treated with tetracycline hydrochloride (1.0 g/kg body weight) were exposed to the extract in a dose of 50 mg/kg for 5 days. On the 7th day of the experiment the following indices were determined: malonic dialdehyde concentration, catalase activity, the levels of ATP, pyruvate and lactate in the liver homogenate, as well as the blood levels of reduced glutathione. The liver pathomorphological investigation was applied. The H. erectum extract was shown to inhibit lipid peroxidation, to increase the activity of the host endogenous antioxidant system, to normalize the hepatocyte energy provision and to limit the liver degeneration.

INFLUENCE BAA «BAIKALSKI - 7» ON CHRONIC TOCSIC / Монголын эм зүй, эм судлал

Introduction.Modern structure of hepatitides etiology and clinical course significantly changed. Prevalence of acute hepatitis tends to reduce, whilst number of patients with chronic hepatitis dramatically rises. Chronic hepatitis fraction is currently extended with mixed forms and lesions caused by toxic agents including drugs.Taking into account this issue, it is necessary to draw attention to the complimentary therapies supplemented with herbal medicines.Our research is focused on measuringpharmacotherapeutic efficiency of«BAIKALSKI - 7»dietary supplement in experimental liver lesions in white rats.Materials and Methods: Dietary supplement «BAIKALSKI - 7» consists of raw material of 7 medicinal plants: calendula flowers(Calendula officinalis L.), rose hips (Rosa majalisHerrm.), thornapple fruits (Grataegussanguinea L.), elecampane rhizomes(Inulahelenium L.), immortelle flowers (Helichrysum are-narium L.),licorice roots(Glicyrrhizaglabra L.)and peppermint leaves(Menthapiperita L.)in equal portions. The composition is based on pharmacological attributes and designed in the Institute of General and Experimental Biology of Siberian Division of the Russian Academy of Sciences(SD RAS). To determine features of «BAIKALSKI - 7» an experimental study in 90 rats of both genders with initial weight ranging from 160,0 to 200,0 was conducted. Chronic toxic hepatitis model was created by subcutaneous injection of CCl4 50% oil solution 0,2ml per 100 g of animal’s weight twice a week during 2 month. All rats were divided into 4 groups: I – intact rats, II –control group with chronicССl4 – hepatitis, III - comparison group with chronic ССl4 – hepatitis received standard treatment drug Carsil(Silibinin50 mg/kg), IV - chronic ССl4 – hepatitis treated with «BAIKALSKI - 7» injected into rats’ stomach. «BAIKALSKI - 7» infusion was used in dosage of dry extract 0,3 g per kgof animal’s weight once a day for 30 days after the 31st day of trials. II group animals received distilled water in equal volume.Functional condition of the liver was measured on 44th, 58th and 72ndday using approved biochemical methods. State of antioxidant defense was rated by means of serum antioxidant enzymes catalase, superoxide dismutases, and glutathione (GSH). Measuring of serum catalase activity is based on salts of ammonium molybdate ability to dye hydrogen peroxide. SOD activity was measured by inhibiting adrenaline autooxidationreaction(рН 10,2). Lipid peroxidation was tracked with malondialdehyde and conjugated dienes concentration in serum. Data is statistically approved forр< 0,05(Student t-test).Results: After injecting hepatotoxic CCl4 model showed increasing cytolytic markers (AST, ALT), cholestasis markers (ALP, ß-lipoprotein, cholesterol) and signs of dysproteinemia(thymol

[Effect of plant extracts from Baikal region on the choleretic reaction in white rats].

In the given work was estimated the choleretic activity of the extracts that was derived from the plants of the Baikal region. It had been shown that the experimental-therapeutic doses of the extracts stimulate choleretic reaction in intact rats.

[The characteristics of the action of polyphytochol on the course of experimental hepatitis and cholecystitis]. / Osobennosti deistviia polifitokhola na techenie éksperimental'nogo gepatita i kholetsistita.

The effect of polyphytochol representing a combination of vegetable agents was studied on models of acute toxic hepatitis caused by CCl4 and cholecystitis. The efficiency of the drug in pathology of the hepatobiliary system was shown: it decreases cholestasis acting as a choleretic and bile-secreting drug, acts on the main pathogenetic links in hepatitis, exerts a marked membrane-stabilizing effect, influences favourably the processes of synthesis in the liver.

[Effect of antihepatotoxic tea on the course of drug-induced hepatitis]. / Vliianie antigepatotoksicheskogo chaia na techenie lekarstvennogo gepatita.

Pharmacotherapeutic efficacy of antihepatoxic tea was studied on an experimental model of tetracycline-induced hepatitis. It was concluded that the tea had a marked pharmacotherapeutic effect on the process of tetracycline-induced hepatitis in animals. It lowered the level of the cytolytic syndrome, prevented the progress of cholestasis and stimulated the bile secretory function of the liver. The favourable effect of the plant antihepatotoxic preparation was due to the presence of a complex of its biologically active substances.

[Effect of kaleflon on the course of experimental hepatitis]. / Vliianie kaleflona na techenie éksperimental'nogo gepatita.

A pronounced cholagogic effect of kaleflon was found in the experiment on albino rats. The course administration of kaleflon in a dose of 0.05 g/kg exerts a marked pharmacotherapeutic action on the course of experimental hepatitis.