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FEMS Microbiol Lett ; 256(2): 251-7, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16499614

RESUMO

Enteroaggregative Escherichia coli (EAEC) is characterized by the expression of the aggregative adherence pattern to cultured epithelial cells. In this study, we determined the phenotypic and genotypic relationships among 86 EAEC strains of human and animal (calves, piglets and horses) feces. Serotypes and the presence of EAEC virulence markers were determined, and these results were associated with ribotyping. Strains harboring aggR (typical EAEC) of human origin were found carrying several of the searched markers, while atypical EAEC harbored none or a few markers. The strains of animal origin were classified as atypical EAEC (strains lacking aggR) and harbored only irp2 or shf. Strains from humans and animals belonged to several different serotypes, although none of them prevailed. Sixteen ribotypes were determined, and there was no association with virulence genes profiles or serotypes. Relationship was not found among the strains of this study, and the assessed animals may not represent a reservoir of human pathogenic typical EAEC.


Assuntos
Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/veterinária , Escherichia coli/genética , Escherichia coli/fisiologia , Virulência/genética , Animais , Bovinos , Doenças dos Bovinos/microbiologia , Impressões Digitais de DNA , DNA Bacteriano/genética , DNA Bacteriano/metabolismo , Escherichia coli/classificação , Escherichia coli/isolamento & purificação , Proteínas de Escherichia coli/genética , Doenças dos Cavalos/microbiologia , Cavalos/microbiologia , Humanos , Proteína 2 Reguladora do Ferro/genética , Epidemiologia Molecular , Hibridização de Ácido Nucleico , Filogenia , Ribotipagem , Sorotipagem , Suínos/microbiologia , Doenças dos Suínos/microbiologia , Transativadores/genética , Fatores de Virulência/genética
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