From green to red: Urban heat stress drives leaf color evolution.

Prevalence of impervious surface and resulting higher temperatures in urban areas, known as urban heat islands, comprises prominent characteristics in global cities. However, it is not known whether and how urban plants adapt to such heat stress. This study focused on Oxalis corniculata, which has intraspecific polymorphism in leaf color (green and red) and examined whether the leaf color variation is associated with urban heat stress. Field observations revealed that green-leaved plants were dominant in green habitats, and red-leaved individuals were dominant in urban habitats, at local (<500 meters), landscape (<50 kilometers), and global scales. Growth and photosynthesis experiments demonstrated that red-leaved individuals performed better under heat stress, while green-leaved individuals performed better under nonstressful conditions. Genome-wide SNP analysis suggests that the red leaf may have evolved multiple times from the ancestral green leaf. Overall, the results suggest that the red leaves of O. corniculata observed in cities worldwide are evidence of plant adaptive evolution due to urban heat islands.

Evolution of competitive traits changes species diversity in a natural field.

Studying the interaction between evolutionary and ecological processes (i.e. eco-evolutionary dynamics) has great potential to improve our understanding of biological processes such as species interactions, community assembly and ecosystem functions. However, most experimental studies have been conducted under controlled laboratory or mesocosm conditions, and the importance of these interactions in natural field communities has not been evaluated. In this study, we focused on the contemporary divergence of a competitive trait (the height-width ratio) of an annual grass Eleusine indica between urban and farmland populations and investigated how trait evolution affects ecological processes by transplanting E. indica individuals from lineages with different trait values into semi-natural grassland. The competitive trait of the transplanted individuals not only affected their own growth and fitness, but also affected the vegetative growth of the competing species and the species diversity. These results indicate that the evolution of competitive traits, even in a single species, can influence the community species diversity through changes in interspecific interactions. Eco-evolutionary interactions therefore play a crucial role in natural field environments. Our results suggest that understanding intraspecific variation in competitive traits driven by rapid evolution is essential for understanding interspecific competitive interactions, community assembly and species diversity.

Disturbance legacy of a 100-year flood event: large wood accelerates plant diversity resilience on gravel-bed rivers.

Understanding biodiversity resilience after a major disturbance is a key issue in basic and applied science. Plant diversity in gravel-bed rivers is affected by flood events, which are one of the most effective disturbance agents in the flow regime, affecting species distribution, and ecosystem dynamics. Although disturbance plays a critical role in community assembly mechanisms, how plant diversity recovers after a severe disturbance, such as a 100-year flood event remains unknown. The present study examined how the disturbance legacy of large wood in gravel-bed river ecosystems contributes to the resilience of plant diversity. The present study demonstrated that the resilience of plant species in disturbance legacy sites, namely deposited large wood sites, was higher than that in open habitat sites. Indicator species analysis revealed that perennial plants were the most important indicator species of disturbance legacy sites. These results suggest that perennial species richness contributes to the resilience of high plant diversity across the disturbance legacy sites in this region. After major flood events, land managers often remove large wood and debris jams to avoid secondary disasters, such as embankment collapse. However, we suggest that large wood should be retained on the gravel beds to aid the recovery of biodiversity and ecosystems. Furthermore, understanding the relationships between disturbance legacies and ecosystem resilience can contribute to the formulation of strategies for sustainable ecosystem management and biodiversity conservation in the future.

GIS-Based Analysis for UAV-Supported Field Experiments Reveals Soybean Traits Associated With Rotational Benefit.

Recent advances in unmanned aerial vehicle (UAV) remote sensing and image analysis provide large amounts of plant canopy data, but there is no method to integrate the large imagery datasets with the much smaller manually collected datasets. A simple geographic information system (GIS)-based analysis for a UAV-supported field study (GAUSS) analytical framework was developed to integrate these datasets. It has three steps: developing a model for predicting sample values from UAV imagery, field gridding and trait value prediction, and statistical testing of predicted values. A field cultivation experiment was conducted to examine the effectiveness of the GAUSS framework, using a soybean-wheat crop rotation as the model system Fourteen soybean cultivars and subsequently a single wheat cultivar were grown in the same field. The crop rotation benefits of the soybeans for wheat yield were examined using GAUSS. Combining manually sampled data (n = 143) and pixel-based UAV imagery indices produced a large amount of high-spatial-resolution predicted wheat yields (n = 8,756). Significant differences were detected among soybean cultivars in their effects on wheat yield, and soybean plant traits were associated with the increases. This is the first reported study that links traits of legume plants with rotational benefits to the subsequent crop. Although some limitations and challenges remain, the GAUSS approach can be applied to many types of field-based plant experimentation, and has potential for extensive use in future studies.

Traditional Ecological Knowledge Maintains Useful Plant Diversity in Semi-natural Grasslands in the Kiso Region, Japan.

Wild plant species provide a variety of ecosystem services that contribute to human well-being. However, much of the legacy of traditional ecological knowledge (TEK) in Japan is rapidly being lost because of environmental changes; therefore, it is important to study the relationship between TEK of ecosystem management practices and plant diversity. Our study area is located in the southwest of Nagano Prefecture, Japan. We compared plant diversity among three land management types including traditional, labor-saving, and land abandonment sites, where we recorded 103 useful plant species based on interviews concerning the traditional use of local ecological resources; among them, 45 species are used for horse livestock, 32 for agriculture, 16 as edible plants, and 19 for manufacture of diverse every-day life goods. Data analyses demonstrated that useful plant diversity was significantly higher in the traditional sites than in other sites. We found highly diverse traditional uses of plant species (e.g., edible plants, horse feed, and rainwear) provided by TEK of local management. These results imply that when local farmers perform traditional management practices, they increase plant species diversity. With our work we investigated the effect of the loss of cultural values and the impact of biodiversity changes on the opportunities that people have to use ecosystem resources in Japan. This aspect particularly highlights the urgency of reconnecting nature and people. Conservation planning based on TEK has been and will be vital in addressing the goal of reducing biodiversity loss on a global scale.

Contrasting effects of land-use changes on herbivory and pollination networks.

Land-use changes, one of the greatest threats to global biodiversity, can cause underappreciated effects on ecosystems by altering the structures of interspecific interaction networks. These effects have typically been explored by evaluating interaction networks composed of a single type of interaction. Therefore, it remains unclear whether the different types of interaction networks sharing the same species respond to the same land-use changes in a similar manner.To compare the responses of herbivory and pollination networks to land-use changes, we investigated both types of interaction networks in seminatural grasslands categorized into three types of agricultural land-use (abandoned, extensively managed, and intensively managed) in a Japanese agricultural landscape. We quantified the structures of the interaction networks using several indices (connectance, evenness, diversity, generality, network specialization, and robustness) and compared them among different land-use types. We conducted piecewise SEM to differentiate the direct and indirect effects of land-use changes on the network structures.Although both land-use changes (abandonment and intensification) led to reduced plant and insect species richness, the structures of herbivory and pollination networks showed different responses to the land-use changes. There was a marked contrast in network generality; while, herbivore species were less generalized (i.e., having fewer host plant species) in fields with land-use intensification, pollinator species were less generalized in abandoned fields.Furthermore, the mechanisms behind the changes in interaction networks were also different between pollination and herbivory networks. The change in herbivory network generality was induced by the decrease in plant species richness, whereas the change in pollination network generality was mainly induced by the effect independent of changes in species richness and composition, which possibly reflect the less number of flowers in shaded environment.The present study demonstrates that agricultural land-use changes affect herbivory and pollination networks in contrasting ways and suggests the importance of assessing multiple types of interaction networks for biodiversity conservation in plant-insect systems. Our results also highlight the underappreciated importance of maintaining habitats with an intermediate intensity of land-use.

Patterns of plant diversity loss and species turnover resulting from land abandonment and intensification in semi-natural grasslands.

Land-use changes cause biodiversity loss in semi-natural ecosystems worldwide. Biotic homogenization has led to biodiversity loss, mainly through declines in species composition turnover. Elucidating patterns of turnover in species composition could enhance our understanding of how anthropogenic activities affect community assembly. Here, we focused on whether the decreasing patterns in plant diversity and turnover of species composition resulting from land-use change vary in two regions. We estimated the species diversity and composition of semi-natural grasslands surrounding paddy fields in satoyama landscapes. We examined the differences in species diversity and composition across three land-use types (abandoned, traditional, and intensified) in two regions (Hyogo and Niigata Prefectures, Japan), which were characterized by different climatic conditions. We then assessed alpha-, beta-, and gamma-diversity to compare the patterns of diversity losses in the two regions as a result of land-use changes. In each region, gamma-diversity was consistently higher in the traditional sites compared to abandoned or intensified sites. The analyses revealed that most of the beta-diversity in traditional sites differed significantly from those of abandoned and intensified sites in both regions. However, the beta-diversity of total and perennial species did not differ between traditional and abandoned sites in the Hyogo region. We noted that the beta-diversity of total and perennial species in intensified sites was much lower than that in the traditional sites of the Niigata region. Overall, the patterns of alpha- and gamma-diversity loss were similar in both study regions. Although the biotic homogenization was caused by intensified land-use in the Niigata region, this hypothesis did not completely explain the loss of biodiversity in the abandoned sites in the Hyogo region. The present study contributes to the growing body of work investigating changes in biodiversity as a result of both biotic homogenization and differentiation in semi-natural ecosystems. Conservationists and policy makers should focus on patterns of species composition responded to land-use changes that continue to increase worldwide.

Historical changes in grassland area determined the demography of semi-natural grassland butterflies in Japan.

Semi-natural grassland areas expanded worldwide several thousand years ago following an increase in anthropogenic activities. However, semi-natural grassland habitat areas have been declining in recent decades due to changes in landuse, which have caused a loss of grassland biodiversity. Reconstructing historical and recent demographic changes in semi-natural grassland species will help clarify the factors affecting their population decline. Here we quantified past and recent demographic histories of Melitaea ambigua (Lepidoptera; Nymphalidae), an endangered grassland butterfly species in Japan. We examined changes in demography over the past 10,000 years based on 1378 bp of mitochondrial COI gene. We then examined changes in its genetic diversity and structure during the last 30 years using nine microsatellite DNA markers. The effective population size of M. ambigua increased about 3000-6000 years ago. In contrast, the genetic diversity and effective population sizes of many populations significantly declined from the 1980s to 2010s, which is consistent with a recent decline in the species population size. Our data suggest that the M. ambigua demography can be traced to changes in area covered by semi-natural grasslands throughout the Holocene.

Colonization and persistence of urban ant populations as revealed by joint estimation of kinship and population genetic parameters.

The decrease in biodiversity due to increasing urbanization has been well documented, but the processes of colonization and maintenance of wildlife populations in urban areas remain poorly understood. We address this issue using 462 individuals from 10 urban populations of the ant Formica japonica in Kobe City, Japan. We sampled workers regardless of colony identity, genotyped them using 6 microsatellite loci, and estimated allele frequencies and genotypes of reproductive individuals, together with other population genetic parameters, by estimating kinship structure using a likelihood method. Estimated genetic diversity and effective size of populations were not associated with environmental parameters, suggesting that populations are unaffected by urbanization. However, effective population sizes were small, and frequent population bottlenecks were detected. These results suggest that urban F. japonica populations are unstable, and the possibility of frequent extinctions and recolonizations in urban habitats. Populations were moderately differentiated without isolation by distance, suggesting a strong dispersal ability that enables colonization of urban habitats. Dispersal was male biased. Collectively, F. japonica was regarded as an urban adapter, which can colonize urban habitats by virtue of its preference for open ground and high dispersal ability but can persist in urban populations for only a short time, showing a tendency as a temporary urban inhabitant.

Tremor is attenuated during walking in essential tremor with resting tremor but not parkinsonian tremor.

We used accelerometry and visual examination by a neurologist to measure the intensity and frequency of hand tremor under resting, postural, writing, and walking conditions among patients with essential tremor with resting tremor (n=11) and Parkinson's disease (n=38). The intensity of essential tremor was markedly attenuated during walking relative to resting. The intensity and frequency of parkinsonian tremors were higher while walking than while resting. We suggest that assessment of the intensity and frequency of tremor during walking is clinically useful for differentiating between essential tremor with resting tremor and parkinsonian tremor, especially in the early stages, when the two conditions are often difficult to distinguish. Parkinsonian tremors are known to be enhanced during walking. Our clinical experience, as well as that of others, suggests that the intensity of essential tremor with resting tremor is markedly attenuated during walking.

Urinary 8-hydroxydeoxyguanosine correlate with hallucinations rather than motor symptoms in Parkinson's disease.

BACKGROUND: Oxidative stress is causally associated with the pathogenesis of Parkinson's disease (PD). Oxygen generates a large amount of reactive oxygen species (ROS). ROS including hydroxyl radicals and H(2)O(2) react with guanine residues in DNA and produce 8-hydroxydeoxyguanosine (8-OHdG). 8-OHdG serves as a biomarker for oxidative stress in various diseases. METHOD: We investigated urinary 8-OHdG levels in 61 PD patients and 28 normal subjects to evaluate the correlation with various clinical features. We quantified disease severity using the Unified Parkinson's Disease Rating Scale for motor symptoms (UPDRS part 3), the Mini-Mental State Examination (MMSE) for mental function, and the Tottori University Hallucination Rating Scale (TUHARS) for quantifying hallucinations. RESULTS: There were significant correlations between 8-OHdG and all the examined parameters, but the partial correlation coefficients excluding contributions of all the other parameters showed that only TUHARS and UPDRS part 3 are significantly related to 8-OHdG. In particular, TUHARS correlates best with urinary 8-OHdG levels. CONCLUSION: The significant correlation between urinary 8-OHdG levels and hallucinations but not with dementia suggests that hallucinations are likely to have unique but unidentified mechanisms that lead to excessive production of 8-OHdG.

Lowered cardiac sympathetic nerve performance in response to exercise in Parkinson's disease.

We examined whether cardiac sympathetic denervation influences the cardiovascular response to exercise in Parkinson's disease (PD). Sixteen patients with PD were divided into two groups, according to their cardiac uptake of (123)I-metaiodobenzylguanidine (denervated group, 10 patients with heart to mediastinum (H/M) ratio < 1.7; innervated group, six patients with H/M ratio > 1.7) and compared changes in blood pressure (BP), heart rate (HR), and cardiac contractility with 13 control subjects during ergometric exercise stress. Velocity index (VI), an indicator of cardiac contractility, was measured using impedance cardiography and recorded every minute. Exercise began at a power output of 20 W for the first 2 min and increased 10 W every 2 min to a maximal intensity of 60 W. All control subjects accomplished the procedure while six patients with PD could not continue after the first minute of 50 W loading. There were no significant differences in BP or HR change between the three groups. However, a significant reduction in VI was observed from the first minute of the 30 W workload in the denervated group compared to the control group. This lowered response continued till 50 W loading and was significantly different to the innervated group at 50 W loading. No significant VI changes were observed between the control and innervated groups throughout the exercise test. Patients with PD with reduced MIBG uptake had a lowered cardiac contractility than innervated subjects during exercise, suggesting that this response represents the impaired exercise capacity of patients with PD with cardiac sympathetic denervation.

NMR study on relationships between reorientational dynamics and phase behaviour of room-temperature ionic liquids: 1-alkyl-3-methylimidazolium cations.

We measured longitudinal and transverse relaxation times (T(1) and T(2)) for (1)H, (13)C-T(1) and (13)C spectra for room-temperature ionic liquids of 1-alkyl-3-methylimidazolium bromide [C(n)mim]Br (n = 2, 3, 4) as a function of temperature. Their values and spectra reveal close relationships between their unique phase behaviours and the dynamics of carbons constituting the cations. Carbons in these cations are classified into groups according to their dynamics, namely imidazolium carbons, an N-methyl carbon, a terminal methyl carbon of the alkyl group and others of the alkyl group. The temperature dependences of T(1) values for these groups differ greatly, resulting in a variation in the characteristic thermal behaviours of the salts. Values of (1)H-T(1) and (13)C-T(1) suggest that some carbons continue to move even in the crystalline and/or solid states. Using (13)C-T(1) data, we also estimated the temperature dependences of the correlation times for the segmental motions of carbons in the liquid states.

The profile of motor unit number estimation (MUNE) in spinal and bulbar muscular atrophy.

OBJECTIVE: Spinal and bulbar muscular atrophy (SBMA) is a lower motor neuron disease caused by the expansion of a trinucleotide CAG repeat in the androgen receptor (AR) gene. The fundamental histopathological finding of this disease is an extensive loss of lower motor neurons in the spinal cord and brainstem. It is, however, difficult to evaluate clinically the degree of motor neuron degeneration, which stresses the need for biomarkers to detect the remaining neuronal function. METHODS: The authors performed motor unit number estimation (MUNE) in 52 patients with SBMA, to investigate whether this method could be a potential biomarker of SBMA, and re-evaluated MUNE 1 year later in a subgroup of the patients. RESULTS: The number of functioning motor units was remarkably reduced in patients with SBMA compared with controls, and was correlated with both ipsilateral grip power and disease duration. A longitudinal analysis demonstrated a further reduction in motor units within 1 year. CONCLUSIONS: The results suggest that MUNE is an electrophysiological parameter that reflects the severity and progression of motor neuron degeneration in patients with SBMA.

Asymmetric allylation of unsymmetrical 1,3-diketones using a BINAP-palladium catalyst.

[reaction: see text] The chiral palladium complex generated in situ from [Pd(eta(3)-allyl)Cl](2) and (R)-BINAP is a good catalyst for the catalytic asymmetric allylation of 1,3-diketones. The reaction provided chiral 2,2-dialkyl-1,3-diketones with 64-89% ee in high yields (13 examples). Enantiomeric excesses are strongly affected by the gamma-substituent of the allylic substrates. A variety of unsymmetrical 1,3-diketones were alkylated with cinnamyl acetate in good enantioselectivities via use of the BINAP-palladium catalyst (77-89% ee).

Formation of 3'-O-beta-galactosyl compounds of 5-bromouridine by Sporobolomyces singularis.

Two new compounds of 5-bromouridine, 3'-O-(beta-D-galactopyranosyl)-5-bromouridine and 3'-O-[beta-D-galactopyranosyl-(1-->4)-beta-D-galactopyranosyl]-5-bromouridine, were found to be selectively formed in a high yield in a culture filtrate of Sporobolomyces singularis, when grown on a medium containing lactose and 5-bromouridine.

Enzymatic preparation of ginsenosides Rg2, Rh1, and F1 from protopanaxatriol-type ginseng saponin mixture.

During investigations on the hydrolysis of a protopanaxatriol-type saponin mixture by various glycoside hydrolases, it was found that two minor saponins, ginsenosides Rg 2 and Rh 1, were formed in high yields by crude beta-galactosidase from Aspergillus oryzae and crude lactase from Penicillium sp., respectively. Moreover, a crude preparation of naringinase from Penicillium decumbens readily hydrolyzed a protopanaxatriol-type saponin mixture to give an intestinal bacterial metabolite, ginsenoside F 1 as the main product. A crude preparation of hesperidinase from Penicillium sp. selectively hydrolyzed ginsenoside Re into ginsenoside Rg 1. This is the first report on the enzymatic preparation of minor saponins, ginsenosides Rg 2 and Rh 1, and of an intestinal bacterial metabolite, ginsenoside F 1, with a high efficiency from a protopanaxatriol-type saponin mixture.