RESUMO
This study was carried out to evaluate the in vitro effect of Musa x paradisiaca stem and leaf against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and/or dichloromethane) of Musa x paradisiaca stem and leaf were tested in vitro on four developmental stages of H. contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI) and adult worm motility assay (AWM). The highly significant (P<0.0001) ability to stop larval development (inhibition >67% for each extract) and the negative effect of the dichloromethane extract of leaf on adult worm motility (43% of inhibition of motility after 24h of incubation) compared to the negative controls, suggest anthelmintic properties of Musa x paradisiaca stem and leaf against H. contortus. The active principles responsible for the activity could be secondary metabolites such as terpenoid and flavonoid compounds present in the leaf and stem of the plant.
Assuntos
Anti-Helmínticos/farmacologia , Haemonchus/crescimento & desenvolvimento , Musa/química , Fitoterapia/métodos , Extratos Vegetais/farmacologia , Animais , Cromatografia em Camada Fina , Relação Dose-Resposta a Droga , Guadalupe , Técnicas In Vitro , Larva/crescimento & desenvolvimento , Folhas de Planta/química , Caules de Planta/química , Análise de RegressãoRESUMO
Three extracts (aqueous, methanolic and dichloromethane) of Manihot esculenta (Cassava) leaf were tested in vitro on four development stages of Haemonchus contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI) and adult worm motility assay (AWM). Compared to the negative control, significant effects (P<0.0001) were observed for the methanolic extract of leaf against larval development (57.6% +/-7.6), with a dose dependent effect. These results suggest that Cassava possess anthelmintic activity against H. contortus. The active principles responsible for the activity could be the terpenoids and condensed tannin compounds present in the leaf.
Assuntos
Anti-Helmínticos/farmacologia , Haemonchus/efeitos dos fármacos , Manihot/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Animais , Anti-Helmínticos/química , Haemonchus/crescimento & desenvolvimento , Óvulo/efeitos dos fármacos , Extratos Vegetais/químicaRESUMO
The seeds of HERNANDIA SONORA L. (Hernandiaceae) yielded eight lignans. Five of them have already been described from other species of the HERNANDIA genus, namely podophyllotoxin ( 1), picropodophyllin ( 2), deoxypodophyllotoxin ( 3), hernandin ( 4), and podophyllotoxin acetate ( 5). 5-Methoxypodophyllotoxin ( 6), 5-methoxypodophyllotoxin acetate ( 7), and a dibenzylbutyrolactone ( 8) are new. Their structures are presented in this publication.
