RESUMO
Oligodeoxynucleotides with RNA cleavage activity 1) were conjugated with amines and peptides by solid phase fragment condensation (SPFC). It was found that 29 mer DNA enzyme conjugated with spermine at its 5'-end showed higher affinity to the target RNA sequence and 40 times higher activity of cleavage than native DNA enzyme. It is also to be noted that conjugate DNA enzymes showed increased resistance against nuclease digestion.
Assuntos
DNA/química , Oligodesoxirribonucleotídeos/química , Sequência de Bases , Catálise , Hibridização de Ácido Nucleico , EsperminaAssuntos
Proteínas Nucleares , Oligonucleotídeos/síntese química , Fatores de Transcrição , Sequência de Aminoácidos , Proteína do Homeodomínio de Antennapedia , DNA Antissenso/síntese química , DNA Antissenso/química , Proteínas de Drosophila/química , Proteínas de Drosophila/genética , Produtos do Gene tat/química , Produtos do Gene tat/genética , Proteínas de Homeodomínio/química , Proteínas de Homeodomínio/genética , Hidróxidos , Indicadores e Reagentes , Dados de Sequência MolecularRESUMO
Development of a novel method for the synthesis of DNA conjugates is described. Oligonucleotides were successfully conjugated with a variety of functional molecules on a solid phase (Solid Phase Fragment Condensation) using an amino, a hydroxyl, a thiol, and a carboxyl group. DNA-peptide conjugate was obtained as a pure from by a single RPHPLC purification approximately in 20% yield. Moreover, it was demonstrated that the present method was effective for the preparation of conjugate molecules, DNA-sugar, DNA-polyamine, DNA-lipid and so on. The study to create new intelligent DNAs by accumulation various biofunctions on the molecule by SPFC is now in progress in our laboratory.
