RESUMO
The nucleotide sequence of the matrixprotein (M) gene of the lapinized rinderpest virus (RPV-L) was determined. The full-length cDNA of the RPV-L M gene is composed of 1460 base pairs and is supposed to contain an open reading frame of 1005 nucleotides encoding on M protein of 335 amino acids. The homology of the predicted amino acid among congeneric morbilliviruses such as RPV Kabete 'O' strain (wild strain of RPV), RPV RBOK strain (vaccine strain of RPV for cattle), measles virus (MV), and canine distemper virus (CDV), is approximately 94%, 93%, 87% and 77%, respectively. In the present study, all coding regions of the RPV-L strain have been determined.
Assuntos
Vírus da Peste Bovina/genética , Proteínas da Matriz Viral/genética , Sequência de Aminoácidos , Sequência de Bases , DNA Viral/química , DNA Viral/genética , Genes Virais , Dados de Sequência Molecular , Filogenia , RNA Viral/química , RNA Viral/genética , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Peste Bovina/virologia , Vírus da Peste Bovina/química , Homologia de Sequência de Aminoácidos , Proteínas da Matriz Viral/químicaRESUMO
Forty Caspian seals were surveyed seroepidemiologically between 1993 and 1998 around the times of mass mortality that occurred in 1997 in the Caspian Sea and seven Baikal seals were also surveyed in 1998. Virus neutralizing tests and ELISA clearly suggested that distemper virus epidemic was caused in Caspian seals before the spring of 1997 and that CDV infection continued to occur in Lake Baikal in recent years.
Assuntos
Anticorpos Antivirais/sangue , Surtos de Doenças/veterinária , Vírus da Cinomose Focina/imunologia , Infecções por Morbillivirus/veterinária , Focas Verdadeiras/virologia , Animais , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática/veterinária , Infecções por Morbillivirus/epidemiologia , Infecções por Morbillivirus/virologia , Federação Russa/epidemiologia , Estudos Soroepidemiológicos , Sibéria/epidemiologiaRESUMO
The seroepidemiological survey of cats conducted in northern part of Taiwan in 1998 revealed that the positive rate of feline immunodeficiency virus (FIV)-infection was 21.9% (7/32) and the rate was much higher than those of previous reports. We succeeded in isolation of three strains of FIV from peripheral blood mononuclear cells of the blood samples. Nucleotide sequences of the env variable V3 to V5 region of the strains revealed that the isolates from distinct areas belong to subtype C. These data together with our previous report (Inada et al. 1997. Arch. Virol., 142: 1459-1467) indicate that FIV subtype C is prevalent in northern part of Taiwan.
