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1.
Chemosphere ; 360: 142387, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38801905

RESUMO

This study was designed to investigate the toxic effects of two frequently used commercial insecticides containing endosulfan and indoxacarb on a freshwater amphipod Gammarus kischineffensis. In this context, the 24, 48, 72 and 96 h LC50 values of these pesticides were determined for G. kischineffensis. Then the histopathological effects of these pesticides on the gill tissues of this species were evaluated. At the end of the study, the 96 h LC50 values of commercial-grade endosulfan and indoxacarb for G. kischineffensis were determined as 1.861 µg L-1 and 20.212 mg L-1, respectively. Histopathologically, the most common histopathological alterations in individuals exposed to sublethal concentrations of commercial-grade endosulfan and indoxacarb were pillar cell hypertrophy resulting in atrophy of the hemocoelic space and hemocytic infiltration. Considering these results, it can be said that commercial-grade endosulfan is extremely and indoxacarb is slightly toxic to G. kischineffensis.


Assuntos
Anfípodes , Endossulfano , Inseticidas , Oxazinas , Poluentes Químicos da Água , Animais , Anfípodes/efeitos dos fármacos , Endossulfano/toxicidade , Inseticidas/toxicidade , Oxazinas/toxicidade , Poluentes Químicos da Água/toxicidade , Brânquias/efeitos dos fármacos , Brânquias/patologia , Dose Letal Mediana
2.
Drug Chem Toxicol ; 43(4): 409-414, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30081650

RESUMO

The present study was aimed to report the toxicity of the chlorpyrifos, an organophosphorus insecticide, with regard to histological and cytological effects through light and transmission electron microscopes in the gills of freshwater fish, Anabas testudineus. The fish were exposed to 0.125 (5% of 96 h LC50), 0.250 (10% of 96 h LC50), and 0.375 mgL-1 (15% of 96 h LC50) of chlorpyrifos for 7, 14, and 21 days for light microscopy and 21 days for transmission electron microscopy. The histological effects were seen in all exposed concentrations of 0.125, 0.250, and 0.375 mgL-1. These effects increased with the increase in chlorpyrifos concentrations and duration of exposure. The main histological effects visible in the gill tissue were fusion of secondary lamellae and epithelial hyperplasia. Other effects included the epithelial hypertrophy, lifting of lamellar epithelium, aneurysm, necrosis, and desquamation of epithelial cells were also reported. Cytological effects included epithelial detachment, large subepithelial space, necrotic cells, apoptotic remnant of cells, the presence of macrophages, swelling of mitochondria in the chloride cells, distension of the tubular system, the presence of some large vacuoles, deposition of excessive mucous, and nucleus abnormalities. This study confirms that varying doses of chlorpyrifos have adverse histological and cytological effects in the gills of A. testudineus.


Assuntos
Clorpirifos/toxicidade , Monitoramento Ambiental/métodos , Brânquias/efeitos dos fármacos , Brânquias/patologia , Praguicidas/toxicidade , Animais , Peixes , Microscopia , Microscopia Eletrônica de Transmissão , Necrose
3.
Environ Toxicol Pharmacol ; 71: 103217, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31284173

RESUMO

Ultrastructural and histopathological reponses in the organs of living organisms are important and useful tools to determine the health condition and the effects of pollutants, such as pesticides, on the organisms. The aim of this study is to determine possible histopathological, cytopathological and ultrastructural alterations in gills of Oreochromis niloticus individuals exposed to 850 µg/L carbaryl standart at 7th, 14th and 21st days with light and electron microscopes. The fish were exposed to carbaryl for 21 days and the histopatological, ultrastructural and cytopathological alterations occuring in the gill tissues of organisms were determined by light, Scanning and Transmission Electron Microscopes (SEM and TEM). At the end of the study, it was observed that carbaryl caused both histopathological and cytopathological changes in the gills of O. niloticus. It has been determined that the most of the pathological changes in the exposed organisms are the metabolic defence reactions.


Assuntos
Carbaril/toxicidade , Ciclídeos , Células Epiteliais/efeitos dos fármacos , Brânquias/efeitos dos fármacos , Inseticidas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Células Epiteliais/ultraestrutura , Brânquias/ultraestrutura , Hiperplasia , Microscopia Eletroquímica de Varredura , Microscopia Eletrônica de Transmissão , Mucosa/efeitos dos fármacos , Mucosa/ultraestrutura
4.
Ecotoxicol Environ Saf ; 147: 749-758, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28942278

RESUMO

Studies addressing the toxicity of pesticides towards non-target organisms focus on the median lethal concentration and biochemical response of individual pesticides. However, when determining environmental risks, it is important to test the combined effects of pesticides, such as insecticides and herbicides, which are frequently used together in agricultural areas. Here we aimed to investigate the toxic effects of the combined use of the herbicide atrazine and the insecticides, endosulfan, indoxacarb, and thiamethoxam on Gammarus kischineffensis. To do this, we tested the activities of oxidative stress, detoxification, and neurotoxicity biomarkers. Compared to atrazine alone, we detected higher glutathione-S-transferase, catalase and superoxide dismutase activities (oxidative stress biomarkers) when atrazine was combined with either endosulfan or indoxacarb. However, higher IBR values were determined in organisms where pesticide mixtures were used according to individual use. Based on these results, mixtures of atrazine and other pesticides may cause synergistic effects and may be evidence of increased toxicity and oxidative stress.


Assuntos
Anfípodes/efeitos dos fármacos , Monitoramento Ambiental/métodos , Herbicidas/toxicidade , Inseticidas/toxicidade , Poluentes do Solo/toxicidade , Anfípodes/enzimologia , Animais , Atrazina/toxicidade , Biomarcadores/análise , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Endossulfano/toxicidade , Dose Letal Mediana , Neonicotinoides/toxicidade , Nitrocompostos/toxicidade , Oxazinas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Tiametoxam , Tiazóis/toxicidade , Fatores de Tempo , Testes de Toxicidade Aguda
5.
Environ Toxicol Pharmacol ; 39(2): 720-6, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25710849

RESUMO

Neonicotinoids are a new group of insecticides, and little is known about their toxicity to nontarget freshwater organisms an potential effects on freshwater ecosystems. The aim of this study is to establish the acute toxicity and histopathological effects of thiamethoxam-based pesticide on the gill tissue of Gammarus kischineffensis. In this study G. kischineffensis samples were exposed to 2.5, 5, 7.5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90 and 100mg/l of commercial grade thiamethoxam for 96 h. The 24, 48, 72 and 96 h LC50 values were determined as 75.619, 23.505, 8.048 and 3.751 mg/l respectively. In histopathological study the individuals were exposed to 0.004, 0.04 and 0.4 mg/l thiamethoxam concentrations for 14 days. The results showed that the most common changes at all doses of thiamethoxam were vacuolization and hemostatic infiltration in the gill tissue of G. kischineffensis.


Assuntos
Anfípodes/efeitos dos fármacos , Inseticidas/toxicidade , Nitrocompostos/toxicidade , Oxazinas/toxicidade , Tiazóis/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Brânquias/efeitos dos fármacos , Brânquias/patologia , Dose Letal Mediana , Neonicotinoides , Tiametoxam
6.
Environ Toxicol Pharmacol ; 34(2): 170-179, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22534510

RESUMO

Deltamethrin, is a commonly used pyrethroid pesticide. Vitamin E is a antioxidant that plays an important role in protecting cells against toxicity by inactivating free radicals generated following pesticides exposure. Therefore, it was evaluated whether deltamethrin induced histopathological changes and nuclear abnormalities using micronucleus test in Oreochromis niloticus, and the possible protective effect of vitamin E against deltamethrin inducing adverse effects in O. niloticus were investigated. Fish was fed with no pesticide+control diet, no pesticide+vitamin E-supplemented diet, 1.45µg/l deltamethrin+control diet, 1.45µg/l deltamethrin+vitamin E-supplemented diet for 30 days. Pesticide and diet quality made an impact on histopathological lesions. In treatments of deltamethrin, group fed with control diet showed much greater damage in comparison with group fed with vitamin E supplemented diet. Vitamin E decreased some histopathological changes induced by deltamethrin, but did not confer complete protection. Deltamethrin treatment has been shown to results in a significant increase in the frequency of micronucleus. However, coadministration of deltamethrin and vitamin E showed decrease in the frequency of micronucleus as compared to deltamethrin treated fish. Our results indicate that, the MN assay and histopathology can be used as bioassays for monitoring pollution in aquatic medium. On the other hand, it was observed that vitamin E decreased the genotoxicity and histopathological changes induced by deltamethrin.


Assuntos
Inseticidas/toxicidade , Nitrilas/toxicidade , Substâncias Protetoras/farmacologia , Piretrinas/toxicidade , Vitamina E/farmacologia , Animais , Ciclídeos , Eritrócitos/efeitos dos fármacos , Eritrócitos/patologia , Brânquias/efeitos dos fármacos , Brânquias/patologia , Fígado/efeitos dos fármacos , Fígado/patologia , Micronúcleos com Defeito Cromossômico/induzido quimicamente , Testes para Micronúcleos
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