Dependence of the postirradiation lymphopenia and hypersegmentation of neutrophil nuclei on the therapeutic irradiation exposure in patients with breast carcinoma--some ways of its use for biological detection of irradiation.

In the work, changes are presented of the numbers of lymphocytes and hypersegmented neutrophils after therapeutic irradiation of women after mastectomy. The values found were evaluated statistically by the decision method. It was found that in the course of irradiation the lymphopenia and increase in the number of neutrophils with hypersegmented cellular nuclei occurred. This contraversory course of the values showed an increasing tendency with increasing exposure. An attempt was made, whether the values found could serve for the biological detection if irradiation, when completing the response of the organism to irradiation based on changes of one parameter by a response from changes of second parameter examined. The values were used as training material for the statistical decision method. The results are represented in the from of a diagram for reading off the magnitude of the damage in practice by stimultaneous evaluation of both parameters. A further approach is shown in the biological dosimetry by simultaneous examination of several independent paramters statistically treated by a suitable method.

An assay of biological dosimetry from the changed number of lymphocytes and hypersegmented neutrophiles evaluated by means of statistical decision method.

The paper presented deals with the statistical utilization of changes in lymphocyte and hypersegmented neutrophiles counts in peripheral blood after irradiation for the biological detection of ionizing radiation. Both followed-up parameters are in a good correlation during simultaneous evaluation of their changes, and they fail during evaluation in various time intervals after irradiation. The changes of two hematological parameters after irradiation, lymphopenia and hypersegmentation of cellular nuclei of granulocytes were therefore utilized. The changes under examination were elaborated in the computer and avaluated by method of regression classification and by discriminatory analysis. This analytical way enables a new conception during the evaluation of individual hematological post-irradiation changes. This study is being continued in several lines. On the one hand, further statistical methods of making the results more precise will be used, on the other hand, other laboratory animals will be tested as well. Last but not least, the best statistical method will be used in an attempt to detect biologically the changes after the therapeutical irradiation in man.