[The diagnostic specificity of the antigen-binding lymphocyte test in Neisseria-induced infections].

Neisseria-induce different infections, but many representatives of this genus are saprophytes. In this connection it is important to evaluate the species, and for N. meningitidis also group specificity of the immunological diagnostics of meningococcal infection and gonorrhea. In this work the specificity of the antigen-binding lymphocyte test was studied in experiments with the immunization of rabbits and the examination of patients. In the tests of indirect rosette-formation and its inhibition the role of antigenic relationships between different pathogenic and nonpathogenic Neisseria was excluded and the species and group specificity of our Neisseria immunoreagents, used in the diagnostics of meningococcal infection and gonorrhea, was proved.

[The improvement of the immunodiagnosis of para-influenza infection based on erythrocyte reagents]. / Sovershestvovanie immunodiagnostiki paragrippoznoi infektsii na osnove éritrotsitarnykh reagentov.

Study of different methods of hemosensitization helped detect the optimum technology for the preparation of parainfluenza (type 3) antigenic erythrocyte diagnosticum. This technology ensures the highest sensitivity, specificity, economy (with respect to viral antigen consumption) and stability of the reagent. Titration of antibodies by means of the new diagnosticum and the detection of parainfluenza cases among children with acute respiratory virus infections, acute and chronic glomerulonephritis have been highly effective, considerably more effective than similar determinations by the hemagglutination inhibition test.

[The modelling of a persistent parainfluenza infection]. / Modelirovanie persistentnoi paragrippoznoi infektsii.

A model of parainfluenza persistent infection was developed in guinea pigs, based on the use of the suckling-adapted virus and an immunosuppressor cyclophosphan. The latter increased the titre of virus and its antigen and duration of their detection in the organs, but the dynamics of these findings was different, and also delayed the time of appearance and slightly prolonged the period of detection of antibody.

[Effect of various infections on the development, course and outcome of glomerulonephritis in children]. / Vliianie nekotorykh infektsii na vozniknovenie, techenie i iskhod ostrogo glomerulonefrita u detei.

Bacteriological and virological tests were made at the disease onset and repeatedly in 100 children (66 boys and 34 girls) aged 2 to 15 years suffering from acute glomerulonephritis. The throat mucus was found to contain streptococci and staphylococci; direct immunofluorescence demonstrated antigens of influenza A1, A3, B virus, of parainfluenza virus, serotypes 1 and 3, of adenovirus and respiratory and syncytial virus; HBs-antigens, specific viral and bacterial antibodies were detected in the blood serum. At the onset of acute glomerulonephritis, 52.5% of the patients were diagnosed to have bacterial infection, 12.5% respiratory viral infection, and 27.5% mixed infection (respiratory viral and bacterial). Besides, 25.5% of the patients showed HBs-antigenemia. HBs-antigenemia and mixed (respiratory viral and bacterial) infection were found to produce an adverse effect on the course and outcome of acute glomerulonephritis. Long-term circulation of some viral antibodies (mostly to parainfluenza-3 antigen) was revealed on repeated studies as was their untoward effect on the outcome of acute glomerulonephritis.

[Immunodiagnostic methods in interpreting an outbreak of respiratory syncytial virus infection]. / Immunodiagnosticheskie metody v rasshifrovke vspyshki raspiratorno-sintsitial'noi virusnoi infektsii.

In the determination of the etiology of the outbreak of respiratory viral diseases, caused mainly by respiratory syncytial (RS) virus, on the basis of the comparison of the results of different laboratory tests and some epidemiological and clinical data high specificity of the detection of RS virus antigen and antibodies to it by means of erythrocyte diagnostica (newly developed antibody and commercial antigenic preparations) has been revealed. In the epidemiological situations the rapid methods of the detection of viral antigen are of prime importance.

[Standardization of the determination of the fimbriae (adhesins) of Citrobacter]. / Standartizatsiia opredeleniia fimbrii (adgezinov) Citrobacter.

Trials of guinea pig formalin-treated red cells have shown their stability for at least 3 years and fitness, equal to that of native cells, for the detection of Citrobacter mannose-sensitive and resistant adhesins in hemagglutination and adhesion tests (red cell binding of bacteria). Comparison of the results of bacterial binding and bacterial agglutination of red cells in tests on slides and in wells has shown that the use of fixed red cells accelerates agglutination and makes it more sensitive, probably at the expense of altering the red cell physicochemical characteristics by fixation. Use of formalin-treated red cells essentially simplifies Citrobacter adhesion determination and makes it possible in any conditions.