RESUMO
The fraction obtained from acidic extract of bovine brain homogenate after several steps of chromatographic purification provokes spontaneous aggressive encounters in rats upon intracerebroventricular injection. The simultaneous long-term raising of electric shock-induced aggression with the suppressing of muricidal and intraspecies aggressive behaviour has been observed. Intravenous and intraperitoneal injections of this fraction induce no behavioural changes in rats. It has been determined that the fraction consists of complex compounds of zinc with various aliphatic amines. A similar or higher behavioural activity has been discovered in series of synthetic complexes of zinc with different ligands, that are suggested for use in modelling any nervous and psychiatric disorders connected with an increased aggression level.
Assuntos
Química Encefálica , Sistema Nervoso/efeitos dos fármacos , Compostos de Zinco/farmacologia , Agressão/efeitos dos fármacos , Animais , Bovinos , Cromatografia em Gel , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Masculino , Ratos , Compostos de Zinco/isolamento & purificaçãoRESUMO
The presented software package allows: 1) to input and edit amino acid sequences; 2) to list aligned amino acid sequences of homologous proteins; 3) pairwise comparison of homologous sequences; 4) construction of phylogenetic trees; 5) comparison of two groups of protein sequences from the same family of homologous proteins; 6) graphic identification of conservative and variable regions of homologous sequences. The stepwise application of the programs allows to study the process of amino acid replacement accumulation during certain intervals of species evolution.
Assuntos
Proteínas/genética , Software , Sequência de Aminoácidos , Animais , Microcomputadores , Dados de Sequência Molecular , Fosfolipases A/genética , Homologia de Sequência do Ácido Nucleico , Venenos de Serpentes/química , SerpentesAssuntos
Agressão/efeitos dos fármacos , Modelos Animais de Doenças , Sistema Nervoso/efeitos dos fármacos , Transtornos Psicóticos Afetivos/induzido quimicamente , Transtornos Psicóticos Afetivos/tratamento farmacológico , Animais , Encéfalo , Bovinos , Depressão Química , Avaliação Pré-Clínica de Medicamentos , Masculino , Ratos , Extratos de TecidosRESUMO
Earlier we found that the immune response and antiviral protection from FMDV can be achieved by immunization with uncoupled FMDV peptides. In a search of approaches to animal protection from FMDV A22 strain we prepared a series of peptides corresponding to the putative antigenic determinants. Synthetic 131-149 and 140-149 sequences afforded 50 to 80% protection, both in the free state and conjugated with keyhole limpet hemocyanin. We believe that the 140-149 segment is so far the smallest peptide capable of eliciting specific antiviral protection without conjugation with a high molecular carrier.
Assuntos
Antígenos Virais/imunologia , Aphthovirus/imunologia , Peptídeos/imunologia , Proteínas Virais/imunologia , Animais , Cromatografia Líquida de Alta Pressão , Febre Aftosa/prevenção & controle , Cobaias , Peptídeos/síntese química , Proteínas Virais/síntese química , Proteínas Estruturais Virais , Vacinas ViraisRESUMO
Fractionated soft gels Sephadex LH-20, Sephadex LH-60, Enzakryl K-2 and semirigid gels - Spheron P-40, Spheron P-300 and Spheron P-1000 were tested for separation of protected synthetic fragments of neurotoxin II from the Central Asian cobra (Naja naja oxiana) venom. Applicability of the above gels for size exclusion HPLC of protected peptides in organic media is discussed.
