[Induction of protein synthesis in mammalian lymphocytes during hyperthermia]. / Induktsiia belkovogo sinteza v limfotsitakh mlekopitaiushchikh pri gipertermii.

Induction of heat shock proteins (HSP's) synthesis in human lymphocytes in vitro was studied using 1D- and 2D-polyacrylamide gel electrophoresis. High resolution of the O'Farrell's procedure revealed the existence of several isoforms of HSP70. The same major HSP's (70 kDa and 88 kDa) were found in rabbit lymphocytes in vivo under whole body hyperthermia. Freshly isolated lymphocytes seem to offer a new approach to the investigation of HSP synthesis induction in mammals under whole body hyperthermia.

[Molecular mechanisms of adaptation to hyperthermia in higher organisms. III. Induction of heat-shock proteins in two Leishmania species]. / Molekuliarnye mekhanizmy adaptatsii k gipertermii u vysshikh organizmov. III. Induktsiia belkov teplovogo shoka u dvukh vidov leishmanii.

The heat-shock proteins (hsp) induction in two species of Leishmania have been investigated. The species studied are parasites of two species of lizards (Lymnodactylus caspins and Agama caucasica) differing by temperature of correspondent ecological niche. Our results show that Leishmania species restricted to high-temperature host (Agama) is capable to synthesize its proteins at extreme temperatures (38, 40 degrees C) with greater intensity. Moreover, the species of Leishmania studied differed by heat-shock proteins pattern, the intensive synthesis of hsp88 and hsp48 being the characteristic features of Leishmania species, restricted to the high-temperature host.

[Circular dichroism of cAMP-dependent protein kinase from pig brain]. / Analiz spektrov krugovogo dikhroizma cAMP-zavisimoi proteinkinazy iz mozga svin'i.

The circular dichroism spectra of phosphorylated and non-phosphorylated forms of cAMP-dependent protein kinase from pig brain and those of the catalytical and regulatory subunits of the enzyme were studied. The percentage of the secondary structure components of the subunits was calculated. cAMP was shown to cause conformational changes of the enzyme. The conformation of the cyclic nucleotide within the cAMP--regulatory subunit complex was established. It was assumed that the conformation of the cAMP molecule during enzyme activation is subjected to inversion.

[Cyclic AMP-dependent pig brain protein kinase: subunit structure, mechanism of autophosphorylation and holoenzyme dissociation under cyclic AMP action]. / cAMP-zavisimaia proteinkinaza iz mozga svin'i: subdedinichnaia struktura, mekhanizm avtofosforilirovaniia i dissotsiatsiia na subdedinitsy pod deistviem cAMP.

Some properties of cyclic AMP-dependent pig brain protein kinase were studied. The holoenzyme was shown to exist in solution in the form of a tetramer complex R2C2 with mol. weight of 180 000. The limited proteolysis of the regulatory subunit caused the formation of a fragment with mol. weight of 35 000, capable of independent binding of 3H-cyclic AMP and containing a site, which can be phosphorylated in the autophosphorylation reaction. Autophosphorylation of the holoenzyme led to an increase in the degree of dissociation of the former into individual subunits under the effect of cyclic AMP. The ability of the phosphoform of the catalytic subunit was demonstrated. The autophosphorylation process and the phosphotransferase reaction involve the same active site of the catalytic subunit.