RESUMO
In vitro experiments have revealed no perceptible decrease in the content of antitoxic antibodies to alphastaphylolysin in the preparations of antistaphylococcal immunoglobulin, containing 13-50% of Fab-fragments. These data confirm the necessity of controlling the molecular composition of the preparations of antistaphylococcal IgG by physico-chemical methods with the aim to evaluate their quality, as the fragmented preparations are rapidly eliminated from a human body.
Assuntos
Especificidade de Anticorpos , Fragmentos Fab das Imunoglobulinas/imunologia , Imunoglobulinas/imunologia , Staphylococcus/imunologia , Proteínas Hemolisinas/análise , Fragmentos Fab das Imunoglobulinas/análise , Imunoglobulinas/análise , Relação Estrutura-AtividadeRESUMO
A study was made of the biological properties of the spore culture of anthrax vaccine strain STI-1 lyophilized in 1944 and kept for 30 years without any passages. This dry culture contained not less than 29% of live spores; the culture growth in the nutrient broth and on the agar medium was typical for the strain. Immunogenicity tested in experiments on guinea pigs and rabbits was not reduced and corresponded to that of the reference STI-1 vaccine.