RESUMO
We studied the molecular mechanisms of cross-adaptation to ionizing radiation (1 Gy) of lymphocytes isolated from rats subjected to emotional stress. The effects of chronic (CES; various types of stress exposure) and acute (AES; forced swimming) emotional stress in rats on indicators of oxidative stress, cell death, and levels of NRF2 and NOX4 proteins involved in the development of the adaptive response were analyzed in isolated lymphocytes. It was found that stress induced an adaptive response in rat lymphocytes and triggered processes similar to the adaptive response induced by low doses of ionizing radiation: an increase in the level of oxidized DNA and cell death, as well as an increase in the content of NOX4 and NRF2 proteins. In animals subjected to emotional stress, suppressed DNA oxidation in response to irradiation, reduced levels of protective factor NRF2, as well as lymphocyte death were observed.
Assuntos
Linfócitos , Fator 2 Relacionado a NF-E2 , Estresse Oxidativo , Radiação Ionizante , Estresse Psicológico , Animais , Linfócitos/efeitos da radiação , Linfócitos/metabolismo , Ratos , Fator 2 Relacionado a NF-E2/metabolismo , Fator 2 Relacionado a NF-E2/genética , Estresse Psicológico/metabolismo , Masculino , Estresse Oxidativo/efeitos da radiação , Ratos Wistar , Adaptação Fisiológica/efeitos da radiação , NADPH Oxidase 4/metabolismo , NADPH Oxidase 4/genética , Dano ao DNA/efeitos da radiaçãoRESUMO
The aims of the present work were to perform a comparative study of the effects of delta sleep-inducing peptide and Deltaran on neurons in emotiogenic brain structures and to address the question of whether it is possible to prevent or decrease the negative influences of stress loads on the severity of subsequent cerebral ischemia in rats, using glycine with delta sleep-inducing peptide combined in the neuroprotective formulation Deltaran. The results showed that Deltaran and delta sleep-inducing peptide had largely the same actions on the nature of spike activity of neurons in the dorsal hippocampus, paraventricular nucleus of the hypothalamus, and ventral anterior nuclei of the thalamus, evoking activation of some of the neurons in these brain structures. The dorsal hippocampus was dominated by activation of spike activity in response to administration of delta sleep-inducing peptide; Deltaran produced activation mainly in the paraventricular nuclei of the hypothalamus. In all animals given Deltaran, the index of brain blood supply was significantly greater than in animals not given Deltaran. The survival rate of cerebral ischemia was 100% in animals given Deltaran. Death occurred in 38% of animals not given Deltaran.
Assuntos
Isquemia Encefálica/prevenção & controle , Encéfalo/efeitos dos fármacos , Peptídeo Indutor do Sono Delta/farmacologia , Glicina/farmacologia , Neurotransmissores/farmacologia , Estresse Psicológico/tratamento farmacológico , Potenciais de Ação/efeitos dos fármacos , Glândulas Suprarrenais/patologia , Animais , Encéfalo/irrigação sanguínea , Encéfalo/fisiopatologia , Isquemia Encefálica/complicações , Isquemia Encefálica/mortalidade , Combinação de Medicamentos , Hipocampo/efeitos dos fármacos , Masculino , Tamanho do Órgão/efeitos dos fármacos , Núcleo Hipotalâmico Paraventricular/efeitos dos fármacos , Ratos , Ratos Wistar , Restrição Física , Estresse Psicológico/complicações , Estresse Psicológico/mortalidade , Estresse Psicológico/fisiopatologia , Tálamo/efeitos dos fármacos , Timo/patologiaRESUMO
The characteristics of the actions of the non-competitive blocker of NMDA receptors dizocilpine on the expression of the early c-fos gene in the paraventricular nuclei of the hypothalamus were studied in rats with different predicted susceptibilities to emotional stress in conditions of treatment with delta sleep-inducing peptide. The results showed that prior treatment with dizocilpine blocked reductions in c-fos expression induced by delta sleep peptide.
Assuntos
Peptídeo Indutor do Sono Delta/fisiologia , Maleato de Dizocilpina/farmacologia , Núcleo Hipotalâmico Paraventricular/metabolismo , Proteínas Proto-Oncogênicas c-fos/biossíntese , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Animais , Peptídeo Indutor do Sono Delta/farmacologia , Regulação da Expressão Gênica , Predisposição Genética para Doença , Injeções Intraperitoneais , Injeções Intraventriculares , Masculino , Núcleo Hipotalâmico Paraventricular/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-fos/genética , Ratos , Ratos Wistar , Estresse Psicológico/genética , Estresse Psicológico/metabolismoRESUMO
Emotional stress induced more marked increases in the expression of the c-fos gene in limbo-reticular structures of the brain in rats prognostically predisposed to emotional stress. I.p. doses of delta sleep-inducing peptide (DSIP) (60 nmol/kg) weakened the stress-induced expression of the c-fos gene. This effect was more apparent in animals predisposed to emotional stress, in which preliminary injections decreased stress-induced c-fos expression in the paraventricular hypothalamus and the medial and lateral parts of the septum. The decreased expression of the early gene c-fos in emotional stress after preliminary dosage with DSIP may reflect the leading mechanism of the anti-stress action of this peptide.
