Three Biannual Rotations Cycles with Residue Incorporation Affect Wheat Production and Chemical Soil Properties.

BACKGROUND: There are few reports of crop rotations with high residue incorporation in terms of their effects on indicator crop yields and soil properties, so this study evaluated the effect of two medium-term biannual rotations on wheat yield development and soil chemical properties after six years of rotation. METHODS: The experiment was conducted with two biannual rotations (canola-wheat and bean-wheat) and four residue incorporation levels (0%, 50%, 100%, and 200%) in an Andisol in south central Chile. Wheat grain yield and residue production were evaluated during each biannual cycle during three cycles of crop rotation, and soil chemical properties were evaluated at final evaluation. RESULTS: The use of beans as a wheat preculture partially improved grain yield in 7.3%. The chemical properties of the soil showed an increase in pH (0.08 units), organic matter content (15 g kg-1), and concentrations of P (2.8 mg kg-1), S (7.4 mg kg-1), and Al (0.03 cmol+ kg-1) after canola cultivation, while after bean cultivation there was an increase in the available N concentration (3.7 mg kg-1). The use of increasing doses of residue allowed for an increase in the soil pH and decrease in the exchangeable Al concentration. CONCLUSION: The continuous incorporation of the residues produced within the biannual rotations evaluated in this volcanic soil did contribute to improving some chemical properties of the soil without affecting wheat crop yield.

Selective ablation of thymic and peripheral Foxp3+ regulatory T cell development.

Foxp3+ regulatory T (Treg) cells of thymic (tTreg) and peripheral (pTreg) developmental origin are thought to synergistically act to ensure immune homeostasis, with self-reactive tTreg cells primarily constraining autoimmune responses. Here we exploited a Foxp3-dependent reporter with thymus-specific GFP/Cre activity to selectively ablate either tTreg (ΔtTreg) or pTreg (ΔpTreg) cell development, while sparing the respective sister populations. We found that, in contrast to the tTreg cell behavior in ΔpTreg mice, pTreg cells acquired a highly activated suppressor phenotype and replenished the Treg cell pool of ΔtTreg mice on a non-autoimmune C57BL/6 background. Despite the absence of tTreg cells, pTreg cells prevented early mortality and fatal autoimmunity commonly observed in Foxp3-deficient models of complete Treg cell deficiency, and largely maintained immune tolerance even as the ΔtTreg mice aged. However, only two generations of backcrossing to the autoimmune-prone non-obese diabetic (NOD) background were sufficient to cause severe disease lethality associated with different, partially overlapping patterns of organ-specific autoimmunity. This included a particularly severe form of autoimmune diabetes characterized by an early onset and abrogation of the sex bias usually observed in the NOD mouse model of human type 1 diabetes. Genetic association studies further allowed us to define a small set of autoimmune risk loci sufficient to promote ß cell autoimmunity, including genes known to impinge on Treg cell biology. Overall, these studies show an unexpectedly high functional adaptability of pTreg cells, emphasizing their important role as mediators of bystander effects to ensure self-tolerance.