SGLT inhibitors as antidiabetic agents: a comprehensive review.

Diabetes is one of the most common disorders that substantially contributes to an increase in global health burden. As a metabolic disorder, diabetes is associated with various medical conditions and diseases such as obesity, hypertension, cardiovascular diseases, and atherosclerosis. In this review, we cover the scientific studies on sodium/glucose cotransporter (SGLT) inhibitors published during the last decade. Our focus on providing an exhaustive overview of SGLT inhibitors enabled us to present their chemical classification for the first time.

Phytochemical analysis of Canna indica L. roots and rhizomes extract.

Canna indica L. (Cannaceae) roots and rhizomes were reported to possess various biological properties like antimicrobial, anthelmintic potential and HIV-1 reverse transcriptase inhibition. In our previous studies, they showed antidiabetic activity on normal rats and rats co-addicted with caffeine and nicotine. In the pursuit of the phytochemical/s responsible for these biological activities, present study was aimed at phytochemical evaluation of hydroalcoholic extract (HAE) of C. indica L. roots and rhizomes; including preliminary screening, thin layer chromatography, H1-NMR and HR-LC/MS-MS analysis. After preliminary detection of flavonoids, tannins and sterols, HAE was tested for presence of ß-sitosterol using TLC. H1-NMR spectrum of HAE revealed the presence of around 761 deshielded protons corresponding to different polar compounds. HR-LC/MS-MS analysis carried out at both positive and negative ion mode, indicated the presence of more than 90 compounds including short fragment of peptide. As per METLIN database, predicted major phytochemicals were 3'-hydroxytrimethoprim, 3,7-epoxycaryophyllan-6-one, swietenine, typhasterol, hexacosanedioic acid and 3ß, 6α,7α-trihydroxy-5ß-cholan-24-oic acid few of which, are biologically active.

Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy.

BACKGROUND: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. OBJECTIVE: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. MATERIALS AND METHODS: Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. RESULTS: Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. CONCLUSIONS: Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin. SUMMARY: Quercetin and rutin were identified from CC roots, PL leaves and BH seeds by liquid chromatography-mass spectroscopy.Quercetin was characterized at (m/z 301) and rutin (m/z 610) as the parent ion peaks.Analogues such as quercetin-O-hexoside, Vicenin 2 and quercetin-3-O-glucoside were identified as fragments.

Structural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by gas chromatography: Mass spectroscopy.

BACKGROUND: Benincasa hispida (BH) and Carissa congesta (CC) are regarded as ethnopharmacological imperative plants in Asian countries. OBJECTIVE: Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in the BH and CC extracts. The presence of lupeol has been reported previously by us using high-performance thin-layer chromatography and high-performance liquid chromatography. MATERIALS AND METHODS: Present research studies encompasses identification of chemical constituents in BH seeds and CC roots petroleum ether extracts by hyphenated technique such as gas chromatography-mass spectroscopy (MS) which when coupled gives a clear insight of constituents. RESULTS: The components were identified by matching mass spectra with MS libraries. There were 13 and 10 different compounds analyzed from CC and BH, respectively. The components present were Pentanoic acid, 5-hydroxy, 2,4-butylphenyl; n-Hexadecanoic acid (Palmitic acid); Sulfurous acid, 2-ethylhexylhepatdecyl ester; n-Tridecane; 6-methyltridecane; (9E, 12E)-9,12-Octadecadienyl chloride, Hexadecanoic acid, 3-(trimethylsilyl)-oxy] propyl ester; 9,12-Octadecadenoic acid, 2 hydroxy-1-(hyroxymethylethyl) ester; 9,12-Octadecadienoic acid, 2,3 dihydroxypropyl ester; n-Propyl-9,12-Octadecadienoate, Lupeol; Taraxasterol; 6a, 14a-Methanopicene, perhydro-12,4a, 61a, 9,9,12a-hepatmethyl-10-hydoxy and 9-Octadecene; 2-Isoprpenyl-5-methyl-6-hepten-1-ol; n-Hexadecanoic acid, 2-hyroxy-1-(hydroxymethyl) ethyl ether; Butyl-9,12-Octadecadieonate; Friedoolean-8-en-3-one; friedours-7-en-3-one; 13,27-Cyclosuran-3-one; Stigmaste-7,25-dien-3-ol (3ß, 5α); Stigmasta-7,16-dien-3-ol; chrondrillasterol in BH seeds and CC roots extracts respectively. CONCLUSION: Eluted components from the extracts could provide further researchers to work with various pharmacological activities related models and studies.