RESUMO
A indicaçäo da presença de residuos de pesticidas organoclorados e organofosforados, em amostras de frutas e hortaliças, foi feita atraves de ensaios biológicos com moscas Drosophila melanogaster (Meig). Simultaneamente, procedeu-se à determinaçäo qualitativa e quantitativa dos resíduos por cromatografia gasosa. Desta maneira, foi possível comparar se os resultados observados no método de bioanálise coincidiam ou näo com os obtidos pelo método químico de cromatografia gasosa. Constatou-se que em 65% do total de 223 amostras analisadas, os resultados foram coincidentes nos dois métodos. Os resultados indicaram que, apesar das limitaçöes, os ensaios biológicos, quando utilizados como método preliminar de triagem podem prestar auxílio na pesquisa de resíduos tóxicos
Assuntos
Cromatografia Gasosa , Frutas , Inseticidas Organoclorados/intoxicação , Inseticidas Organofosforados/intoxicação , Plantas , Inseticidas Organoclorados/análise , Inseticidas Organofosforados/análise , PraguicidasRESUMO
The cytogenetic effect of DDT on human blood cultures, in vitro, was investigated. Two types of experiment were carried out: one, in which the DDT concentrations found in the culture media were similar to those found in the plasma of individuals of the Brazilian population (0.06--0.20 mug/ml); in the second experiments, doses ranging from 1 to 15 mug/ml were used. No correlation was found between DDT doses and cells with chromosomal aberrations. The Poisson test of comparison between means showed that at certain DDT concentrations (0.20, 4.05 and 8.72 mug/ml) the proportion of cells with structural aberrations was significantly greater than in the controls.
Assuntos
Aberrações Cromossômicas , Cromossomos/efeitos dos fármacos , DDT/farmacologia , Aneuploidia , Relação Dose-Resposta a Droga , Linfócitos/ultraestrutura , PoliploidiaRESUMO
Workers from three insecticide plants in direct contact with 2,2-bis (beta-chlorophenyl)-I,I,I,-trichloroethane (DDT) did not differ significantly in the frequencies of cells with chromosomal aberrations when compared with controls from the same plants but not in direct contact with the drug. The same was true when a group of workers from one plant was compared with a control group from the Instituto Butantan, with no history of occupational exposure to DDT. Yet, when the control group from one of the three plants, which showed high DDT plasmic levels, was added to the group in direct contact with the insecticide, the frequency of cells with chromatid aberrations was significantly higher, suggesting that DDT causes chromatid lesions. A positive correlation was found between DDT levels and times of exposure, but being in direct or indirect contact with DDT was not always correlated with the degree of contamination.
Assuntos
Aberrações Cromossômicas/efeitos dos fármacos , DDT/efeitos adversos , Doenças Profissionais/induzido quimicamente , Adulto , Cromossomos/efeitos dos fármacos , DDT/sangue , Exposição Ambiental , Humanos , Cariotipagem , Linfócitos/ultraestrutura , Masculino , Fatores de TempoRESUMO
Workers from three insecticide plants in direct contact with 2,2-bis (â-chloro-phenyl)-1,1,1,-trichloroethane (DDT) did not differ significantly in the frequencies of cells with chromosomal aberrations when compared with controls from the same plants but not in direct contact with the drug. The same was true when a group of workers from one plant was compared with a control group from the Institute Butantan, with no history of occupational exposure to DDT. Yet, when the control group from one of the three plants, which showed high DDT plasmic levels, was added to the group in direct contact with the insecticide, the frequency of cells with chromatid aberrations was significantly higher, suggesting that DDT causes chromatid lesions. A positive correlation was found between DDT levels and times of exposure, but being in direct or indirect contact with DDT was not always correlated with the degree of contamination.
