[BIOLOGICAL PROPERTIES OF SALMONELLA, ISOLATED FROM CLINICAL MATERIAL AND AQUATIC ENVIRONMENT IN ROSTOV REGION].

AIM: Study biological properties of salmonella, isolated from clinical materials and water of Don river. MATERIALS AND METHODS: Salmonella strains of various serovars were used in the study. Biochemical characteristics were studied by generally accepted methods, antigenic properties were evaluated in agglutination reactions, virulence was determined by Dlm for laboratory animals, antibiotics sensitivity was verified by disc-diffusion method. RESULTS: The presence of pathogenicity factors in isolated strains was shown: hemolytic activity--in 64 and 36.8% of cases, DNAse activity--in 28 and 26%, respectively in clinical and wild strains. Microorganism dose, resulting in death of all the animals (LD100) did not depend on serovar of salmonella and varied from 10(3) to 10(10) PFU/ml. CONCLUSION: Clinical strains were established to possess higher virulence and resistance to antibiotics compared with strains isolated from the aquatic envionment.

[Crimean-Congo hemorrhagic fever in Rostov Province: the epidemiological characteristics of an outbreak]. / Krymskaia-Kongo gemorragicheskaia likhoradka v Rostovskoi oblasti: épidemiologicheskie osobennosti vspyshki.

The results of the epidemiological analysis of the outbreak of hemorrhagic fever which was caused by Crimean-Congo hemorrhagic fever virus and occurred during the period of July 3-19, 1999, in the Oblivskaya district of Rostov Province are presented. The specific epidemiological features of the outbreak have been determined. The possible versions of the appearance of the focus of infection and the role of Ixodes ticks in the circulation of the infective agent are discussed.

[Epidemiologic characterization of Brucellosis under modern conditions]. / Epidemiologicheskaia kharakteristika brutseleza v sovremennykh usloviiakh.

The spread of brucella infection under modern conditions of cattle breeding were studied with the foci in the Rostov region taken as an example. The main epidemiological features and tendencies of epidemiological importance were established. To find out specific antigen in different environmental objects, the approbation of the enzyme immunoassay test systems was carried out and the possibility of their use at the institutions of the State Sanitary and Epidemiological inspection was shown.

[Effect of indirect endolymphatic antibiotic therapy on clinical and immunological indicators in patients with erysipelas of the lower extremities]. / Vliianie nepriamoi éndolimfaticheskoi antibiotikoterapii na klinicheskie i immunologicheskie pokazateli u bol'nykh rozhei nizhnikh konechnostei.

Two groups of patients with erysipelas of the lower extremities underwent indirect endolymphatic therapy with bicillin-3 (58 patients) and routine penicillin therapy (79 patients). Comparative clinicoimmunological examinations in the two groups revealed that lymphotropic administration of the antibiotic had a more favourable effect on the disease process, as evidenced by more rapid reverse time courses of erysipelas clinical signs, less frequent incidence of early relapses and normalization of the majority of immunological parameters by the end of the treatment. For estimation of the anti-recurrence efficacy of the antibiotic therapy in the patients with erysipelas, it was recommended to use a specific scale based on the principles of the Wald successive alternative analysis.

[Changes in the kallikrein-kinin system of patients with erythematous and hemorrhagic forms of erysipelas]. / Izmenenie kallikrein-kininovoi sistemy u bol'nykh s éritematoznoi i gemorragicheskoi formoi rozhi.

The state of the kallikrein-kinin system of the blood (KKS): kallikrein (KK), prekallikrein (PK), general arginine-esterase activity (GAEA), alpha 1 inhibitor of protease (IP) and alpha 2-macroglobulin (MG). The initial period of the disease was characterized by an increase of the level of KK, GAEA, IP and MG and a reduction of PK. It was established that the maximal activation of KKS develops in patients with the erythematous form of erysipelas during the first days of the disease, with the hemorrhagic form--on the 2 week. During convalescence KKS indices showed the reverse dynamics but did not reach normal levels in most patients. It is suggested that there is a relationship of the "rapid" form of KKS activation with formation of erythematous erysipelas, the "slow"--with hemorrhagic erysipelas. It is recommended to use the KKS indices, primarily KK activity for prediction of hemorrhagic erysipelas at the early prehemorrhagic stage.

[The pathogenetic and prognostic significance of the level of class-E immunoglobulins in erysipelas]. / Patogeneticheskoe i prognosticheskoe znachenie urovnia immunoglobulinov klassa E pri rozhe.

The authors determined the content of total immunoglobulins of the E class in the blood plasma: native (IgE) and exhausted by a streptococcal allergen in the dynamics of the disease in 145 patients with erysipelas. The level of antistreptococcal reagins was evaluated. It was established that at the onset of erysipelas the concentration of IgE and a-IgE showed a short-term increase in 52 +/- 4% and 87 +/- 3% of patients correspondingly. During convalescence the level of these antibodies did not differ from that of the normal excluding high risk patients for recurrence. The participation of IgE-mediated type of allergic reactions in the pathogenesis of erysipelas is suggested. It is recommended to use the content of IgE and a-IgE for the prognosis of immediate and remote recurrences of erysipelas.

[The use of the kallikrein-kininogen system indices in the prognosis of the development of hemorrhagic erysipelas]. / Ispol'zovanie pokazatelei kallikrein-kininovoi sistemy dlia prognoza razvitiia gemorragicheskoi formy rozhi.

A number of the kallikrein-kinin system parameters (kallikrein, prekallikrein, total arginine esterase activity, alpha 1 protease inhibitor, and alpha 2 macroglobulin) were measured in 59 patients with erythematous erysipelas and in 51 ones with hemorrhagic erysipelas over the course of the disease. Marked activation of the blood kallikrein-kinin system was seen in all the patients during the initial period of the disease, manifesting by elevated levels of kallikrein, total arginine esterase activity, alpha 1 protease inhibitor, alpha 2 macroglobulin, and a lowered prekallikrein concentration. In erythematous erysipelas the peak of activation was recorded in the first days of the disease, whereas in hemorrhagic condition it was observed during the second week of erysipelatous inflammation. Different patterns of changes in the kallikrein-kinin system over the course of the disease permit using one of its parameters, kallikrein activity, for the prediction of the development of local hemorrhagic syndrome in erysipelas patients already during the earliest (prehemorrhagic) stage of the condition.

[Characteristics of the course of diphtheria in alcohol abuse]. / Osobennosti techeniia difterii u zloupotrebliaiushchikh spirtnymi napitkami.

A comparative study of the diphtheria course was conducted in 46 patients who denied taking alcohol or who used it not more than once a month and in 21 patients who used alcohol continuously. A more severe course, complicated forms of diphtheria and fatal outcomes were more frequently encountered in the latter group of patients.

[A rare case of the meningoencephalitis form of the Loeffler syndrome]. / Redkii sluchai meningoéntsefaliticheskoi formy sindroma Lefflera.

The article describes a case of benign meningoencephalitis attended by marked eosinophilia. The disease was evaluated as a meningoencephalitic form of Loeffler's syndrome. Drug-induced allergy is considered as a possible cause of the disease.