RESUMO
Cell contact with hyaluronic acid as a component of the extracellular matrix plays an important role in the development of many physiological and pathological processes. In experiments on laboratory rats, the model of chronic pancreatitis was reproduced. Prolonged occlusion of the pancreatic duct caused the pancreatic acinar cells atrophy and pancreatic fibrosis. It was found that the development of chronic pancreatitis accompanied by an increase of concentrations of hyaluronic acid in plasma as a marker of fibrosis of the pancreas. The redistribution of hyaluronate-binding activity of the receptor in the pancreas and the brain: cerebellum, hippocampus and thalamus were observed. The most pronounced redistribution of degree of affinity to hyaluronate was observed in the membrane fraction of proteins: in the cerebellum and thalamus the sensitivity to this glycosaminoglycan was decreased, while in the hippocampus the sensitivity was increased, which may serve as a signal for some adaptive processes in these parts of the brain under the influence of factors that accompany the development of chronic pancreatitis.
Assuntos
Proteínas de Transporte/metabolismo , Cerebelo/metabolismo , Hipocampo/metabolismo , Ácido Hialurônico/metabolismo , Pâncreas/metabolismo , Pancreatite Crônica/metabolismo , Tálamo/metabolismo , Animais , Membrana Celular/metabolismo , Modelos Animais de Doenças , Fibrose , Masculino , Pâncreas/patologia , Pâncreas/ultraestrutura , Pancreatite Crônica/patologia , RatosRESUMO
In experiment on laboratory rats the models of acute and chronic pancreatitis were developed to study the changes of lipoperoxidation-antioxidant protection system depending on morphological changes of the pancreas. The acute and chronic pancreatitis is accompanied with intensification of lipoperoxidation and gradual inhibition of antioxidant system due to development of subsequent chronization of the pathological process.
Assuntos
Glutationa Peroxidase/sangue , Glutationa Redutase/sangue , Glutationa/sangue , Pancreatite/sangue , Doença Aguda , Animais , Modelos Animais de Doenças , Masculino , Malondialdeído/sangue , Pancreatite/patologia , Pancreatite Crônica/sangue , Pancreatite Crônica/patologia , Ratos , Índice de Gravidade de DoençaRESUMO
A probable increase of the level of calcium-binding protein S100b and soluble form of glial fibrillary acidic protein (GFAP), as well as reducing the level of filament GFAP in the brain of Wistar rats under hepatic encephalopathy development caused by chronic hepatitis (HP) were shown. Increasing concentrations of S100b may stimulate the disassembly of intermediate filaments of astrocytes. The immunohistochemical analysis helps to reveal that astrocytes in the brain of rats that had HP lose the characteristic stellate shape and swelling. Immunoblotting result have shown the fragmentation of the main filament form of GFAP and appearance of low mass derivates. Application of 2-oxoglutarate (2.28 g/l of drinking water during 10 days after the onset of chronic hepatitis) stabilized the studied proteins and the state of astroglia.
