Inactivation kinetics modeling of Escherichia coli in concentrated urine for implementing predictive environmental microbiology in sanitation safety planning.

Urine concentration (condensation) leads to the inactivation of pathogens in urine owing to a hyperosmotic environment. This study proposed an inactivation kinetic model of Escherichia coli (E. coli), a surrogate of human bacterial pathogens, in concentrated synthetic urine. The model parameters were obtained under an assumption that the inactivation rate of E. coli followed a binomial distribution, which made it possible to accurately simulate the time-course decay of E. coli in synthetic urine. The inactivation rate constant values obtained in concentrated urine samples, ammonium buffer solutions and carbonate buffer solutions indicated that the osmotic pressure was a relatively predominant cause for the inactivation of E. coli. The appropriate storage time was estimated using the approach of quantitative microbial risk assessment, which indicated that the 5-fold concentrated urine could be safely collected after 1-day storage when urea was hydrolyzed, whereas 91-hour storage was required for non-concentrated urine. The occupational risk was not negligible even with 6-month storage at 20 °C when urea was not hydrolyzed, which suggested that the urine storage styles should be clarified more minutely. The present study highlights the importance of "predictive environmental microbiology," which deals with inactivation kinetic models of microorganisms under varied environmental conditions to fully implement the hazard analysis and critical control point (HACCP) approach for the safe use of human excreta in agriculture.

Bactericidal and virucidal mechanisms in the alkaline disinfection of compost using calcium lime and ash.

In the present study, the bactericidal and virucidal mechanisms in the alkaline disinfection of compost with calcium lime and ash were investigated. Two indicator microorganisms, Escherichia coli and MS2 coliphage, were used as surrogates for enteric pathogens. The alkaline-treated compost with calcium oxide (CaO) or ash resulted primarily in damage to the outer membrane and enzyme activities of E. coli. The alkaline treatment of compost also led to the infectivity loss of the coliphage because of the partial capsid damage and RNA exteriorization due to a raised pH, which is proportional to the amount of alkaline agents added. These results indicate that the alkaline treatment of compost using calcium oxide and ash is effective and can contribute to the safe usage of compost from a mixing type dry toilet.

Grey water treatment by the slanted soil system with unsorted soil media.

This study evaluated the performance of unsorted soil media in the slanted soil treatment system, in terms of removal efficiency in suspended solids (SS), chemical oxygen demand (COD), linear alkylbenzene sulphonate (LAS) and Escherichia coli, and lifetime until clogging occurs. Unsorted soil performed longer lifetime until clogging than sorted fine soil. Removal of SS, COD, and LAS also performed same or better level in unsorted soil than fine soil. As reaction coefficients of COD and LAS were described as a function of the hydraulic loading rate, we can design a slanted soil system according to the expected hydraulic loading rate and the targeted level of COD or LAS in effluent. Regarding bacteria removal, unsorted soil performed sufficient reduction of E. coli for 5 weeks; however, the removal process occurred throughout all four chambers, while that of fine soil occurred in one to two chambers.

Suitability of biochar as a matrix for improving the performance of composting toilets.

To evaluate the suitability of biochar (rice husk charcoal) as a matrix in composting toilets that can decompose human faeces and recover fertiliser components, the composting process during toilet operation and the agricultural value of the resulting compost were characterised by performing a comparison with sawdust, rice husks, and corn stalks. The faecal decomposition ratio in biochar was 42%, similar to the values for rice husks (46%) and corn stalks (41%), but higher than the value for sawdust (25%). Heterotroph micro-organism acidity is qualitatively higher in biochar than in sawdust. However, nitrogen loss in biochar was 19%, lower than that in rice husks (36%) and corn stalks (25%), but similar to that in sawdust (16%). Although the biochar compost had no significant impact on the cation exchange capacity and water retention of sandy soil, the ratio of nitrogen transportation into plants was 12.8%, higher than that for the other materials. These results suggest that biochar is effective for achieving high faecal decomposition, low nitrogen loss, and high nutrient supply.