Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Saudi J Gastroenterol ; 20(2): 120-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24705150

RESUMO

BACKGROUND/AIM: We aimed to assess the influence of Helicobacter pylori and its virulent factors, cytotoxin associated gene (cag) A and E, on portal hypertensive gastropathy (PHG) and the levels of interleukin (IL)-8, IL-10, and tumor necrosis factor-alpha (TNF-α). PATIENTS AND METHODS: The patients with cirrhosis underwent screening endoscopy and the lesions related to PHG were graded. Biopsies were obtained for histology, and polymerase chain reaction (PCR) of H. pylori 16S rRNA, cagA, cagE, and tissue cytokine levels was carried out. Absent or mild PHG was compared with moderate to severe PHG. RESULTS: One hundred and forty patients with cirrhosis were studied; males numbered 92 and the mean age of the patients was 50.3 ± 12.0 years, H. pylori positivity in 87 (62.1%) patients was associated with male gender (P = 0.032), younger age (P = 0.029), hepatitis D etiology (P = 0.005), higher serum albumin (0.000), lower Child Pugh score (P = 0.001), and lower portal vein diameter (P = 0.001). There was no significant difference in the levels of TNF-α and IL-8. However, a decrease in the anti-inflammatory cytokine IL-10 was noted with moderate to severe gastropathy. Four H. pylori strains were positive for both cagA and cagE, while four were positive for cagA only. All the four patients with both virulent factors had mild gastropathy only. CONCLUSION: The presence of H. pylori infection neither affected the severity of PHG nor augmented the IL-8 and TNF-α levels. There was a decline of virulent H. pylori strains and IL-10 levels in patients with advanced PHG.


Assuntos
Infecções por Helicobacter , Helicobacter pylori , Hipertensão Portal/complicações , Interleucina-10/sangue , Interleucina-8/sangue , Gastropatias/sangue , Gastropatias/etiologia , Fator de Necrose Tumoral alfa/sangue , Adulto , Idoso , Antígenos de Bactérias/análise , Proteínas de Bactérias/análise , Endoscopia Gastrointestinal , Feminino , Infecções por Helicobacter/sangue , Infecções por Helicobacter/diagnóstico , Helicobacter pylori/patogenicidade , Humanos , Hipertensão Portal/microbiologia , Hipertensão Portal/patologia , Masculino , Pessoa de Meia-Idade , Gastropatias/diagnóstico , Gastropatias/microbiologia , Gastropatias/patologia , Virulência
2.
Eur J Gastroenterol Hepatol ; 26(6): 630-9, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24722560

RESUMO

INTRODUCTION: This preliminary study aimed to investigate the effects of the probiotic Saccharomyces boulardii on proinflammatory and anti-inflammatory cytokines in patients with diarrhea-dominant irritable bowel syndrome (IBS-D). The other objectives were to document any clinical improvement as judged by symptoms, quality of life, and histology. PATIENTS AND METHODS: This was a randomized, double blind, placebo-controlled trial in which S. boulardii, 750 mg/day, or placebo was administered for 6 weeks in IBS-D patients, in addition to ispaghula husk standard treatment. RESULTS: Thirty-seven patients received S. boulardii and 35 patients received the placebo. As compared with placebo, the S. boulardii group showed a significant decrease in blood and tissue levels of proinflammatory cytokines interleukin-8 (IL-8) and tumor necrosis factor-α (P<0.001) and an increase in anti-inflammatory IL-10 levels, as well as an increase in the tissue IL-10/IL-12 ratio (P<0.001). No significant change in the blood and tissue levels of cytokines was found in the placebo group. Bowel-related IBS-D symptoms reported in the patients' daily diary improved in both groups. However, overall improvement in the quality of life was more marked in the S. boulardii group. Although baseline histological findings were mild, an improvement was observed in the probiotic group in the lymphocyte and neutrophil infiltrates (P=0.017 and 0.018), epithelial mitosis (P=0.003), and intraepithelial lymphocytes (P=0.024). No serious adverse events were found in either group. CONCLUSION: S. boulardii with ispaghula husk was superior to placebo with ispaghula husk in improving the cytokine profile, histology, and quality of life of patients with IBS-D. These preliminary results need to be confirmed in a well-powered trial.


Assuntos
Citocinas/metabolismo , Diarreia/terapia , Síndrome do Intestino Irritável/terapia , Probióticos/uso terapêutico , Saccharomyces , Adulto , Colo/imunologia , Colo/patologia , Diarreia/etiologia , Diarreia/imunologia , Diarreia/patologia , Método Duplo-Cego , Feminino , Humanos , Mucosa Intestinal/imunologia , Mucosa Intestinal/patologia , Síndrome do Intestino Irritável/complicações , Síndrome do Intestino Irritável/imunologia , Síndrome do Intestino Irritável/patologia , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Probióticos/efeitos adversos , Psicometria , Qualidade de Vida , Resultado do Tratamento , Adulto Jovem
3.
Saudi J Gastroenterol ; 19(5): 211-8, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24045594

RESUMO

BACKGROUND/AIM: Helicobacter pylori is a Gram-negative bacteria, which is associated with development of gastroduodenal diseases. The prevalence of H. pylori and the virulence markers cytotoxin-associated gene A and E (cagA, cagE) and vacuolating-associated cytotoxin gene (vacA) alleles varies in different parts of the world. H. pylori virulence markers cagA, cagE, and vacA alleles in local and Afghan nationals with H. pylori-associated gastroduodenal diseases were studied. PATIENTS AND METHODS: Two hundred and ten patients with upper gastrointestinal symptoms and positive for H. pylori by the urease test and histology were included. One hundred and nineteen were local nationals and 91 were Afghans. The cagA, cagE, and vacA allelic status was determined by polymerase chain reaction. RESULTS: The nonulcer dyspepsia (NUD) was common in the Afghan patients (P = 0.025). In Afghan H. pylori strains, cagA was positive in 14 (82%) with gastric carcinoma (GC) compared with 29 (45%) with NUD (P = 0.006), whereas cagE was positive in 11 (65%) with GC and 4 (67%) with duodenal ulcer (DU) compared with 12 (18%) with NUD (P < 0.001 and 0.021, respectively). The vacA s1a/b1was positive in 10 (59%) of GC compared with 20 (31%) in NUD (P = 0.033). In Pakistani strains, cagE was positive in 12 (60%) with GC, 7 (58%) with GU, 12 (60%) with DU compared with 11 (16%) with NUD (P < 0.001, 0.004, and < 0.001, respectively). In Pakistani strains, cagA/s1a/m1 was 39 (33%) compared with Afghans in 17 (19%) (P = 0.022). Moderate to severe mucosal inflammation was present in 51 (43%) Pakistani patients compared with 26 (28%) (P = 0.033) in Afghans. It was also associated with grade 1 lymphoid aggregate development in Pakistani patients 67 (56%) compared with 36 (40%) (P = 0.016) in Afghans. CONCLUSION: Distribution of H. pylori virulence marker cagE with DU was similar in Afghan and Pakistan H. pylori strains. Chronic active inflammation was significantly associated with Pakistani H. pylori strains.


Assuntos
Antígenos de Bactérias/genética , Proteínas de Bactérias/genética , Úlcera Duodenal/genética , Infecções por Helicobacter/genética , Helicobacter pylori/patogenicidade , Adulto , Afeganistão/epidemiologia , Estudos de Coortes , Neoplasias Duodenais/epidemiologia , Neoplasias Duodenais/genética , Neoplasias Duodenais/patologia , Úlcera Duodenal/epidemiologia , Úlcera Duodenal/patologia , Feminino , Regulação da Expressão Gênica , Genótipo , Infecções por Helicobacter/epidemiologia , Infecções por Helicobacter/patologia , Helicobacter pylori/genética , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Paquistão/epidemiologia , Reação em Cadeia da Polimerase , Estudos Retrospectivos , Medição de Risco , Neoplasias Gástricas/epidemiologia , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Úlcera Gástrica/epidemiologia , Úlcera Gástrica/genética , Úlcera Gástrica/patologia , Virulência/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...