RESUMO
The presence of HBV DNA in the blood serum of 50 children with HBsAg-positive chronic hepatitis (36 with chronic hepatitis B, 14 with chronic hepatitis delta) was determined by molecular hybridization method. No significant differences were observed between the frequency of HBV DNA detection and clinicomorphological type of chronic HBsAg-positive hepatitis. In chronic HBV infection, HBV DNA was shown to be detected more frequently than in chronic hepatitis delta. A correlation was confirmed between the frequency of HBV DNA detection and demonstration of HBeAg in the blood. With the increase in the duration of the disease and age of the patients the HBV DNA detection rate was found to diminish.
Assuntos
DNA Viral/sangue , Antígenos de Superfície da Hepatite B/sangue , Hepatite B/sangue , Hepatite Crônica/sangue , Hepatovirus/genética , Adolescente , Envelhecimento/sangue , Criança , Pré-Escolar , Hepatite B/imunologia , Anticorpos Anti-Hepatite B/sangue , Antígenos E da Hepatite B/sangue , Antígenos E da Hepatite B/imunologia , Hepatite Crônica/imunologia , Humanos , Lactente , Cirrose Hepática/sangue , Cirrose Hepática/imunologia , Hibridização de Ácido NucleicoRESUMO
The Saccharomyces cerevisiae DBY 746/pNMVG-20 strain produces HBsAg similar in its properties to HBsAg from hepatitis B patients' plasma. The paper analyses variants of extraction of HBsAg from yeast cells, presents data on immunogenic potency of yeast HBsAg in mice, and describes gel filtration of yeast extracts containing HBsAg.
Assuntos
Regulação da Expressão Gênica , Antígenos de Superfície da Hepatite B/genética , Saccharomyces cerevisiae/genética , Animais , Anticorpos Antivirais/análise , Fenômenos Químicos , Físico-Química , Cromatografia em Gel , Antígenos de Superfície da Hepatite B/análise , Antígenos de Superfície da Hepatite B/imunologia , Imunização , Camundongos , Plasmídeos , Recombinação Genética , Saccharomyces cerevisiae/imunologiaRESUMO
The method of molecular hybridization using cloned DNA of hepatitis B virus as a probe demonstrated the presence of virus-specific DNAs in sera of former blood donors, HBsAg carriers. Among HBsAg-positive patients with anemias, hepatitis B virus DNA was detected in half of the examined subjects which in all cases was accompanied by the presence of HBeAg in their blood.