AGE AND GENDER-SPECIFIC FEATURES OF CYTOGENETIC CHANGES IN BUCCAL EPITHELIUM IN INDIVIDUALS RESIDING IN THE «SICK BUILDING¼.

OBJECTIVE: The aim: The study of cytomorphological and cytogenetic features of the buccal epithelium of residents of apartments who complained of unpleasant odors in their homes. PATIENTS AND METHODS: Materials and methods: The state of buccal epithelium in residents of multi-story buildings was studied. A total of 237 individuals were examined, 117 males and 120 females, aged from 6 to 81 years. Buccal cells were collected using a sterile spatula and stained with a 2.5% solutionofaceto-orcein and 1% light green. The preparations were examined using a light microscope OPTON Axioskop (Germany) with oil immersion at a magnification of x1000. Statistical processing of the data was performed using IBMSPSS Statistics 29.0.0.0 (t-Student criterion; Mann-Whitney; ANOVA: Tukey; T3-Dunnett), with p≤0.05. RESULTS: Results: Cytomorphological and cytogenetic abnormalities, compared to physiological limits, were mainly manifested as karyorrhexis, nuclear doubling, the appearance of epitheliocytes with perinuclear vacuoles, or nuclear vacuolization. The frequency of micronuclei was observed in the range of (0.3-2.8 ‰). The highest micronucleus index (per 1000 cells, ‰) was observed among males aged 15-39 years and females over 65 years old. In both sexes, the lowest micronucleus indices were found in the age group of 6-14 years. CONCLUSION: Conclusions: in the «sick building¼ an increase in the frequency of micronucleus occurrence among males and females was observed simultaneously with increasing age.

ASSESSMENT OF THE CONSEQUENCES OF THE DETERIORATION OF THE EPIDEMIOLOGICAL SITUATION DURING HOSTILITIES.

OBJECTIVE: The aim: Scientific substantiation of the methodology for predicting the consequences of the worsening of the epidemic situation on the territory of Ukraine during military operations for the timely adoption of measures for the medical protection of military personnel in conditions of biological contamination. PATIENTS AND METHODS: Materials and methods: Determination and generalization of the impact of biological contamination due to the use of biological weapons were carried out considering the main determinants of the epidemic process using the index and coefficient of medical protection. Applied methods of scientific research: epidemiological, system, and information approach. RESULTS: Results: The authors proposed indicators that consider the pathogenicity of the infectious agent, contagiousness, the degree of non-specific protection of servicemen, specific protection of servicemen, and the sanitary-epidemiological state of the area of operations of troops (forces). Relevant epidemic situations were simulated, and the index and coefficient of medical protection were calculated to predict the consequences of the worsening of the epidemic situation to make timely decisions regarding the implementation of medical protection measures for military personnel in conditions of biological contamination during the repulsion of armed aggression. CONCLUSION: Conclusions: In the conditions of biological contamination, when biological weapons and biological terrorism are used, the epidemic process in the army is intensified, which requires timely decisions regarding the implementation of medical protection measures for military personnel in conditions of biological contamination.

ON THE NEED TO IMPROVE THE SYSTEM OF PREVENTION OF ALCOHOL AND DRUG ADDICTION AMONG SERVICEMEN OF THE DEFENSE FORCES.

OBJECTIVE: The aim: Analysis of the legal framework for the development of scientifically sound measures for the prevention and detection of drug addiction and substance abuse in the Armed Forces of Ukraine as an important element of the system of medical support for combat readiness of troops. PATIENTS AND METHODS: Materials and methods: The normative documents on carrying out obligatory preventive narcological examinations in the Armed Forces of Ukraine and other power ministries and departments are considered. CONCLUSION: Conclusions: Today in Ukraine it is necessary to improve the legal framework and strengthen control over the work of military commissariats to study conscripts, collect the necessary information about alcohol and drug addiction of citizens who are called up to the Armed Forces of Ukraine.

RESULTS OF RESEARCH OF PERSONAL HYGIENE PRODUCTS FOR SUITABILITY FOR SPECIAL PROCESSING IN ZONES OF CHEMICAL, RADIOACTIVE CONTAMINATION AND IN COMBAT CONDITIONS.

OBJECTIVE: The aim: Research of personal hygiene products with detoxification properties by evaluating their formulation composition, emulsifying and complexing ability to heavy metal ions and radionuclides. PATIENTS AND METHODS: Materials and methods: The complex-forming ability of raw materials with heavy metal ions was determined in vitro, the content of heavy metals was determined by the method of atomic emission spectrometry with inductively coupled plasma (BS EN 13805:2014); determined the content of 137Cs, 232Th, 40K, 226Ra, 90Sr in washings from the surface of the skin of hands treated with personal hygiene products. The means were tested for compliance with the standard on scintillation spectrometers of gamma radiation energy «SEG-001¼ «AKP-S¼ and beta radiation (SEB-01-70); methods used: MI 12-04-099 and MI 12-05-099. RESULTS: Results: A high complexing ability of the pectin-containing «Liana¼ shampoo was found in relation to heavy metal ions (Co2+, Cu2+, Zn2+, Cd2+, Pb2+), as well as in their combined presence. It was established that the effectiveness of removing fatty impurities from the surface of the skin of the «Liana¼ product was 10.1 times higher than that of liquid soap (p<0.001). A high level of deactivating, degassing and antibacterialproperties of the personal care products «Bastion¼ and «Bastion-M¼ was revealed. CONCLUSION: Conclusions: Today, there is significant potential in the creation of formulations for special (sanitary) skin and hair surface treatment, containing natural complexing agents as detoxicants, and can be used in special professional activities associated with the risk of exposure to CBRN factors.