RESUMO
The physical and chemical parameters of red blood cells were studied in patients with urinary tract infection. The permeability parameters were proposed to study erythrocyte membrane permeability. Complete recovery of the latter in patients with somatic diseases due to antibacterial therapy was found to suggest that the preservation of erythrocyte dysfunction.
Assuntos
Permeabilidade da Membrana Celular/fisiologia , Membrana Eritrocítica/fisiologia , Eritrócitos/química , Infecções Urinárias/diagnóstico , Adolescente , Criança , Pré-Escolar , Humanos , Infecções Urinárias/sangueRESUMO
The behaviour of cefazolin in aqueous and biological media with varying the antibiotic concentration was studied by UV-spectroscopy. Range of the contents and the detection limits of cefazolin were determined. The procedure of UV-spectroscopy of cefazolin in mixed saliva of patients with urinary tract infection was developed.
Assuntos
Antibacterianos/análise , Cefazolina/análise , Saliva/química , Espectrofotometria Ultravioleta/métodos , Infecções Urinárias/tratamento farmacológico , Adolescente , Criança , Humanos , Limite de DetecçãoRESUMO
A method for spectroscopic determination of ceftriaxone in saliva of healthy subjects and patients with upper respiratory tract infections was developed. The concentration range is 1-50 mcg/ml. The method is express and simple, and can be used to determine ceftriaxone in clinical tests and pharmacokinetic studies. Possible determination of ceftriaxone in aqueous solutions was demonstrated which is useful in analysis of drugs.
Assuntos
Ceftriaxona/análise , Saliva/química , Ceftriaxona/farmacocinética , Humanos , Infecções Respiratórias/tratamento farmacológico , Espectrofotometria UltravioletaRESUMO
The paper is devoted to the key problems of etiology and pathogenesis of chronic pyelonephritis in children. Factors responsible for the development of primary and secondary pyelonephritis are discussed. Including urinary tract obstruction. The main stages of pathogenesis are considered with reference to the mechanisms of uropathogenic action of bacterial agents of pyelonephritis.
Assuntos
Pielonefrite/microbiologia , Pielonefrite/fisiopatologia , Bactérias/classificação , Bactérias/isolamento & purificação , Criança , Doença Crônica , Humanos , Pielonefrite/diagnósticoRESUMO
Stability of aqueous solutions of beta-lactam antibiotics (sodium benzylpenicillin, oxacillin, amoxycillin, phenoxymethylpenicillin, cefotaxime, cefazolin) in biological matter of various acidity was investigated spectrophotometrically. Potentiometric sensors (based on ionic association of beta-lactam tetradecylammonium) sensitive to penicillins and cephalosporins within the concentrations of 1 x 10(-5) - 1 x 10(-1) M were designed. The sensors provided determination of concentration of individual antibiotics and their summary content in the presence of a significant excess of inorganic ions. Express methods for ionometric determination of beta-lactams in blood serum and mixed saliva were developed.
Assuntos
Antibacterianos/análise , beta-Lactamas/análise , Sangue , Potenciometria/métodos , Saliva/química , Sensibilidade e EspecificidadeRESUMO
The spectrum method is applied to the study of erythrocytic membrane permeability of water muddiness. A hundred forty patients aged 9-16 and 17-31 years who had quinsy were examined. Before treatment, the changes in the physical and chemical parameters of erythrocytes were identical in the patients with quinsy in both age groups and accompanied by the increased water contents in erythrocytes and their decreased concentration of dry matter. During and after treatment, all parameters of the physical and chemical properties of red blood cells were higher in 17-31-year-old patients than those in 9-16-year olds. The technique for estimating cell dispersion suspensions, which is based on the modern colloidal optics theory, is a reliable one for studying the permeability of cells in the body.