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1.
Biochem Mol Biol Int ; 38(4): 829-38, 1996 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-8728113

RESUMO

A synthetic flavocytochrome with the reductase and oxygenase activities was obtained by covalent binding of riboflavin to cytochrome P450 2B4. The reactions catalyzed by the newly synthesized flavocytochromes were studied. Formation of carboxycomplex with the reduced form of hemoprotein led to 60-80% inhibition of oxygenase reactions indicating the leading role of reduced heme iron in generating active oxygen species by flavocytochromes.


Assuntos
Hidrocarboneto de Aril Hidroxilases , Sistema Enzimático do Citocromo P-450/metabolismo , Riboflavina/metabolismo , Esteroide Hidroxilases/metabolismo , Compostos de Anilina/metabolismo , Animais , Grupo dos Citocromos c/metabolismo , Ferricianetos/metabolismo , Hidroxilação , Masculino , Metilação , NAD/metabolismo , NADP/metabolismo , Oxirredutases/metabolismo , Oxigenases/metabolismo , Coelhos , Espectrofotometria
2.
Gene ; 124(1): 13-9, 1993 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-7916706

RESUMO

A 2648-bp fragment from the P4 plasmid of Shigella sonnei strain 47 coding for the SsoII restriction endonuclease (ENase) and methyltransferase (MTase) (recognition sequence 5'-CCNGG) was sequenced. Two divergently arranged open reading frames of 905 bp for the SsoII ENase (R.SsoII) and 1137 bp for the MTase (M.SsoII) were identified. The coding regions are separated by 110 bp. The calculated M(r) of R.SsoII (35937) and M.SsoII (42887) are in good agreement with values previously obtained by in vitro transcription-translation experiments, i.e., 35 and 43 kDa for the ENase and MTase, respectively. The M.SsoII amino acid (aa) sequence revealed a considerable similarity to m5C-MTases recognizing the related sequences--M.EcoRII, M.dcm, M.MspI, M.BsuFI, M.HpaII, and M.HhaI. Surprisingly, the greatest degree of homology has been observed between the aa sequences of M.SsoII and M.NlaX, with an unidentified recognition sequence. The multiple alignment of aa sequences helps to identify the blocks of conserved aa in variable regions of MTases. These conserved aa can play a key role in target recognition. Some aspects of evolution of m5C-MTases are discussed.


Assuntos
DNA-Citosina Metilases/genética , Desoxirribonucleases de Sítio Específico do Tipo II/genética , Genes Bacterianos , Shigella sonnei/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Códon/genética , Enzimas de Restrição do DNA/genética , DNA Bacteriano/genética , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Escherichia coli/enzimologia , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Dados de Sequência Molecular , Filogenia , Plasmídeos , Homologia de Sequência de Aminoácidos , Shigella sonnei/genética , Especificidade por Substrato , Transcrição Gênica
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