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1.
Wiley Interdiscip Rev RNA ; 14(4): e1776, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36717357

RESUMO

Biomolecular condensates are membrane-less assemblies of proteins and nucleic acids. Centrosomes are biomolecular condensates that play a crucial role in nuclear division, cytoskeletal remodeling, and cilia formation in animal cells. Spatial omics technology is providing new insights into the dynamic exchange of spliceosome components between the nucleus and the centrosome/cilium. Intriguingly, centrosomes are emerging as cytoplasmic sites for information storage, enriched with RNA molecules and RNA-processing proteins. Furthermore, growing evidence supports the view that nuclear spliceosome components assembled at the centrosome function as potential coordinators of splicing subprograms, pluripotency, and cell differentiation. In this article, we first discuss the current understanding of the centrosome/cilium complex, which controls both stem cell differentiation and pluripotency. We next explore the molecular mechanisms that govern splicing factor assembly and disassembly around the centrosome and examine how RNA processing pathways contribute to ciliogenesis. Finally, we discuss numerous unresolved compelling questions regarding the centrosome-associated spliceosome components and transcript variants within the cytoplasm as sources of RNA-based secondary messages in the regulation of cell identity and cell fate determination. This article is categorized under: RNA-Based Catalysis > RNA Catalysis in Splicing and Translation RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes RNA Processing > Splicing Regulation/Alternative Splicing RNA Processing > RNA Processing.


Assuntos
Centrossomo , Spliceossomos , Animais , Spliceossomos/metabolismo , Centrossomo/metabolismo , Proteínas/metabolismo , Splicing de RNA , RNA/metabolismo
2.
Cells ; 11(23)2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36497189

RESUMO

The centrosome consists of two centrioles surrounded by pericentriolar material [...].


Assuntos
Centríolos , Centrossomo
3.
Cells ; 9(10)2020 09 24.
Artigo em Inglês | MEDLINE | ID: mdl-32987651

RESUMO

The centrosome, which consists of two centrioles surrounded by pericentriolar material, is a unique structure that has retained its main features in organisms of various taxonomic groups from unicellular algae to mammals over one billion years of evolution. In addition to the most noticeable function of organizing the microtubule system in mitosis and interphase, the centrosome performs many other cell functions. In particular, centrioles are the basis for the formation of sensitive primary cilia and motile cilia and flagella. Another principal function of centrosomes is the concentration in one place of regulatory proteins responsible for the cell's progression along the cell cycle. Despite the existing exceptions, the functioning of the centrosome is subject to general principles, which are discussed in this review.


Assuntos
Centrossomo/metabolismo , Citoesqueleto de Actina/metabolismo , Animais , Corpos Basais/metabolismo , Centríolos/metabolismo , Centríolos/ultraestrutura , Centrossomo/ultraestrutura , Humanos , Microtúbulos/metabolismo , Microtúbulos/ultraestrutura , Modelos Biológicos
4.
Cells ; 8(2)2019 02 19.
Artigo em Inglês | MEDLINE | ID: mdl-30791486

RESUMO

During spermiogenesis, the proximal centriole forms a special microtubular structure: the centriolar adjunct. This structure appears at the spermatid stage, which is characterized by a condensed chromatin nucleus. We showed that the centriolar adjunct disappears completely in mature porcine spermatozoa. In humans, the centriolar adjunct remnants are present in a fraction of mature spermatids. For the first time, the structure of the centriolar adjunct in the cell, and its consequent impact on fertility, were examined. Ultrastructural analysis using transmission electron microscopy was performed on near 2000 spermatozoa per person, in two patients with idiopathic male sterility (IMS) and five healthy fertile donors. We measured the average length of the "proximal centriole + centriolar adjunct" complex in sections, where it had parallel orientation in the section plane, and found that it was significantly longer in the spermatozoa of IMS patients than in the spermatozoa of healthy donors. This difference was independent of chromatin condensation deficiency, which was also observed in the spermatozoa of IMS patients. We suggest that zygote arrest may be related to an incompletely disassembled centriolar adjunct in a mature spermatozoon. Therefore, centriolar adjunct length can be potentially used as a complementary criterion for the immaturity of spermatozoa in the diagnostics of IMS patients.


Assuntos
Centríolos/metabolismo , Fertilidade/fisiologia , Espermatogênese/fisiologia , Adulto , Animais , Centríolos/ultraestrutura , Cromatina/metabolismo , Humanos , Infertilidade Masculina/patologia , Masculino , Espermátides/metabolismo , Espermátides/ultraestrutura , Suínos , Doadores de Tecidos
5.
J Nanobiotechnology ; 14(1): 67, 2016 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-27576904

RESUMO

BACKGROUND: A new type of superparamagnetic nanoparticles with chemical formula Fe7C3@C (MNPs) showed higher value of magnetization compared to traditionally used iron oxide-based nanoparticles as was shown in our previous studies. The in vitro biocompatibility tests demonstrated that the MNPs display high efficiency of cellular uptake and do not affect cyto-physiological parameters of cultured cells. These MNPs display effective magnetocontrollability in homogeneous liquids but their behavior in cytoplasm of living cells under the effect of magnetic field was not carefully analyzed yet. RESULTS: In this work we investigated the magnetocontrollability of MNPs interacting with living cells in permanent magnetic field. It has been shown that cells were capable of capturing MNPs by upper part of the cell membrane, and from the surface of the cultivation substrate during motion process. Immunofluorescence studies using intracellular endosomal membrane marker showed that MNP agglomerates can be either located in endosomes or lying free in the cytoplasm. When attached cells were exposed to a magnetic field up to 0.15 T, the MNPs acquired magnetic moment and the displacement of incorporated MNP agglomerates in the direction of the magnet was observed. Weakly attached or non-attached cells, such as cells in mitosis or after cytoskeleton damaging treatments moved towards the magnet. During long time cultivation of cells with MNPs in a magnetic field gradual clearing of cells from MNPs was observed. It was the result of removing MNPs from the surface of the cell agglomerates discarded in the process of exocytosis. CONCLUSIONS: Our data allow us to conclude for the first time that the magnetic properties of the MNPs are sufficient for successful manipulation with MNP agglomerates both at the intracellular level, and within the whole cell. The structure of the outer shells of the MNPs allows firmly associate different types of biological molecules with them. This creates prospects for the use of such complexes for targeted delivery and selective removal of selected biological molecules from living cells.


Assuntos
Técnicas Citológicas/métodos , Nanopartículas de Magnetita/química , Microscopia de Fluorescência/métodos , Linhagem Celular Tumoral , Citoesqueleto/química , Citoesqueleto/metabolismo , Endocitose/fisiologia , Humanos , Espaço Intracelular/química , Magnetismo , Microscopia Eletrônica de Transmissão
6.
Bioarchitecture ; 6(3): 47-52, 2016 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-27093502

RESUMO

The centrosome is a key component of the cell is involved in the processes of cell division, cell motility, intracellular transport, organization of the microtubules (MT) network and the production of cilia and flagella. The peculiarity of this organelle is that its boundaries are not clearly defined, the centrioles at the center of the centrosome are surrounded by electron dense pericentriolar material, the size and protein composition of this centrosome component experiences significant transformation during the cell cycle. It has been shown in this study that within the centrosome different proteins occupy different areas corresponding to: MT nucleation region (defined as gamma-tubulin-stained area), regulatory region (defined as kinase pEg2-stained area) and motor proteins region (kinesin-like motor XlEg5-stained area). The boundary of pEg2 is near 1.3 times greater while XlEg5 is 3.0 times greater than that of gamma-tubulin. Thus, the size of the centrosome, determined according to the structural electron microscopy (EM) analysis (about 1 µm) corresponds to the regulatory proteins area, but the actual functional centrosome size defined at the motor proteins region, is more than twice the size.


Assuntos
Centrossomo , Microtúbulos/metabolismo , Ciclo Celular , Centríolos , Mitose
7.
Toxicol Lett ; 208(1): 36-40, 2012 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-22008730

RESUMO

Zinc chloride (0.01 mM kept for 3h) is not toxic to cultured cerebellar granule neurons (CGNs) while kainate (0.1mM kept for 3h) demonstrates some but very low toxicity towards these cells. Measurements of the relative intraneuronal zinc ion concentration showed that increase in [Zn(2+)](i) under the simultaneous action of ZnCl(2) and kainate was significantly stronger compared to their separate action. Simultaneous treatment of CGNs with kainate and zinc chloride caused the swelling of neuronal mitochondria and consequent intensive neuronal death, which was totally prevented by NBQX (an AMPA/kainate-receptors blocker) or ruthenium red (a mitochondrial Ca(2+) uniporter blocker). These data imply that Zn(2+) synergistically to kainate increase their separate toxic effects on mitochondria leading to rapid neuronal death.


Assuntos
Cerebelo/efeitos dos fármacos , Cloretos/toxicidade , Agonistas de Aminoácidos Excitatórios/toxicidade , Ácido Caínico/toxicidade , Mitocôndrias/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Compostos de Zinco/toxicidade , Zinco/toxicidade , Animais , Cálcio/metabolismo , Células Cultivadas , Cerebelo/citologia , Sinergismo Farmacológico , Ratos , Ratos Wistar , Zinco/metabolismo
8.
Neurosci Lett ; 461(2): 140-4, 2009 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-19500653

RESUMO

We found that 60-min glucose deprivation leads to progressive decrease in the mitochondrial membrane potential and increase in [Ca(2+)](i) in cultured cerebellar granule neurons. The latter effect was fully reversible, returning to the basal level 60 min after restoration of normal glucose level in the incubation medium, whereas mitochondrial membrane potential remained at 10.0+/-1.8% below the initial value. Electron microscopy indicated that glucose deprivation induced appearance of mitochondria with local lightening of the matrix and destruction of cristae. This mitochondrial conformation was preserved during the restoration phase after glucose level in the cultivation medium returned to the normal level. Neuronal death within a 24-h period after 60-min glucose deprivation was relatively small, being 14.0+/-4.4%.


Assuntos
Cerebelo/citologia , Glucose/deficiência , Mitocôndrias/fisiologia , Neurônios/fisiologia , Animais , Cálcio/metabolismo , Células Cultivadas , Potencial da Membrana Mitocondrial , Mitocôndrias/ultraestrutura , Neurônios/ultraestrutura , Ratos , Ratos Wistar
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