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1.
Electrophoresis ; 44(1-2): 313-322, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-35315940

RESUMO

Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates.


Assuntos
Microfluídica , Espectrometria de Massas por Ionização por Electrospray , Humanos , Espectrometria de Massas por Ionização por Electrospray/métodos , Microfluídica/métodos , Eritrócitos , Dispositivos Lab-On-A-Chip
2.
Analyst ; 145(23): 7718-7723, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-32996917

RESUMO

We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme ß-galactosidase and substrate 4-methylumbelliferyl ß-d-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.


Assuntos
Corantes Fluorescentes , Microfluídica , Galactose , beta-Galactosidase/genética
3.
J Sep Sci ; 42(1): 445-457, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30444312

RESUMO

Resistive pulse sensing is a well-known and established method for counting and sizing particles in ionic solutions. Throughout its development the technique has been expanded from detection of biological cells to counting nanoparticles and viruses, and even registering individual molecules, e.g., nucleotides in nucleic acids. This technique combined with microfluidic or nanofluidic systems shows great potential for various bioanalytical applications, which were hardly possible before microfabrication gained the present broad adoption. In this review, we provide a comprehensive overview of microfluidic designs along with electrode arrangements with emphasis on applications focusing on bioanalysis and analysis of single cells that were reported within the past five years.


Assuntos
Técnicas Analíticas Microfluídicas/instrumentação , Análise de Célula Única/instrumentação , Eletrodos , Desenho de Equipamento , Humanos , Tamanho da Partícula
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