RESUMO
This study aimed to evaluate the genomic profile of the Antarctic marine Curtobacterium sp. CBMAI 2942, as well as to optimize the conditions for chitinase production and antifungal potential for biological control. Assembly and annotation of the genome confirmed the genomic potential for chitinase synthesis, revealing two ChBDs of chitin binding (Chi C). The optimization enzyme production using an experimental design resulted in a 3.7-fold increase in chitinase production. The chitinase enzyme was identified by SDS-PAGE and confirmed through mass spectrometry analysis. The enzymatic extract obtained using acetone showed antifungal activity against the phytopathogenic fungus Aspergillus sp. series Nigri CBMAI 1846. The genetic capability of Curtobacterium sp. CBMAI 2942 for chitin degradation was confirmed through genomic analysis. The basal culture medium was adjusted, and the chitinase produced by this isolate from Antarctica showed significant inhibition against Aspergillus sp. Nigri series CBMAI 1846, which is a tomato phytopathogenic fungus. This suggests that this marine bacterium could potentially be used as a biological control of agricultural pests.
Assuntos
Antifúngicos , Quitinases , Proteômica , Quitinases/metabolismo , Quitinases/genética , Quitinases/farmacologia , Antifúngicos/farmacologia , Regiões Antárticas , Proteômica/métodos , Genômica/métodos , Aspergillus/enzimologia , Aspergillus/genética , Genoma Bacteriano , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/genética , Organismos Aquáticos , Quitina/farmacologia , Quitina/metabolismo , Quitina/químicaRESUMO
This study aimed to evaluate the genomic profile of the Antarctic marine Curtobacterium sp. CBMAI 2942, as well as to optimize the conditions for chitinase production and antifungal potential for biological control. Assembly and annotation of the genome confirmed the genomic potential for chitinase synthesis, revealing two ChBDs of chitin binding (Chi C). The optimization enzyme production using an experimental design resulted in a 3.7-fold increase in chitinase production. The chitinase enzyme was identified by SDS-PAGE and confirmed through mass spectrometry analysis. The enzymatic extract obtained using acetone showed antifungal activity against the phytopathogenic fungus Aspergillus sp. series Nigri CBMAI 1846. The genetic capability of Curtobacterium sp. CBMAI 2942 for chitin degradation was confirmed through genomic analysis. The basal culture medium was adjusted, and the chitinase produced by this isolate from Antarctica showed significant inhibition against Aspergillus sp. Nigri series CBMAI 1846, which is a tomato phytopathogenic fungus. This suggests that this marine bacterium could potentially be used as a biological control of agricultural pests.
RESUMO
Datos estadísticos nacionales revelan una seroprevalencia de 0,27 por ciento para anti-VIH; 0,92 por ciento para VHBs-Ag; 4,53 por ciento antiVHBc y 0,77 por ciento para la enfermedad de Chagas. Sabemos además que en países como Inglaterra la seroprevalencia para anti-VIH es de 0,001 por ciento y para VHBsAg de 0,1 por ciento. Considerando que la gran diferencia entre Venezuela e Inglaterra es el tipo de donante, siendo relacionado o altruista respectivamente. este trabajo estudia retrospectivamente si la calidad del donante influye en la seroprevalencia de los diversos componentes sanguíneos, analizando los datos existentes en el Banco de Sangre. De un total de 48.124 donantes 242 fueron donantes altruistas y 47.882 donantes relacionados, solo se encontraron diferencias para el anti-VHBc y la seroprevalencia positiva total, es decir, los donantes relacionados poseen una seroprevalencia positiva mayor y constituyen un grupo de mayor riesgo en comparación a los donantes altruistas