[Unconventional hip arthroplasty for a 15-year-old benign bone fibrous histiocytoma in a pediatric patient]. / Artroplastía no convencional de cadera por histiocitoma fibroso benigno óseo en un paciente pediátrico con 15 años de evolución.

INTRODUCTION: Benign fibrous histiocytoma is a rare tumor with very stringent radiologic and histopathologic criteria. It accounts for approximately 1% of all the benign bone tumors. It may be located in both the soft tissues and bone. Its usual location is the shaft, the epiphyseal end of the long bones, the pelvis and the ribs. The age range of presentation is very broad, from 5 to 75 years; it occurs more frequently in young adults. It is rare in children. CASE: Female, 27 year-old patient who presented at 10 years of age with a tumor in the right proximal femur, which was diagnosed as benign fibrous histiocytoma. She underwent surgical en bloc resection and unconventional arthroplasty with a RIMAG prosthesis. She did fine, had no alterations of implant instability and bone quality. She is developing properly, with symmetry of the extremities and appropriate mobility. DISCUSSION: There are only a few articles addressing subtotal and total hip arthroplasty in pediatric ages. There are reports in adolescents ranging from infection to loosening, periprosthetic fracture, revision and, generally speaking, implant failure. However, we did not see this type of complications in this case, and the patient received appropriate management.

Improved soybean root association of N-starved Bradyrhizobium japonicum.

In this study, we addressed the effects of N limitation in Bradyrhizobium japonicum for its association with soybean roots. The wild-type strain LP 3001 grew for six generations with a growth rate of 1.2 day(-1) in a minimal medium with 28 mM mannitol as the carbon source and with the N source [(NH(4))(2)SO(4)] limited to only 20 microM. Under these conditions, the glutamine synthetase (GS) activity was five to six times higher than in similar cultures grown with 1 or 0.1 mM (NH(4))(2)SO(4). The NtrBC-inducible GSII form of this enzyme accounted for 60% of the specific activity in N-starved rhizobia, being negligible in the other two cultures. The exopolysaccharide (EPS) and capsular polysaccharide (CPS) contents relative to cell protein were significantly higher in the N-starved cultures, but on the other hand, the poly-3-hydroxybutyrate level did not rise in comparison with N-sufficient cultures. In agreement with the accumulation of CPS in N-starved cultures, soybean lectin (SBL) binding as well as stimulation of rhizobial adsorption to soybean roots by SBL pretreatment were higher. The last effect was evident only in cultures that had not entered stationary phase. We also studied nodC gene induction in relation to N starvation. In the chromosomal nodC::lacZ fusion Bj110-573, nodC gene expression was induced by genistein 2.7-fold more in N-starved young cultures than in nonstarved ones. In stationary-phase cultures, nodC gene expression was similarly induced in N-limited cultures, but induction was negligible in cultures limited by another nutrient. Nodulation profiles obtained with strain LP 3001 grown under N starvation indicated that these cultures nodulated faster. In addition, as culture age increased, the nodulation efficiency decreased for two reasons: fewer nodules were formed, and nodulation was delayed. However, their relative importance was different according to the nutrient condition: in older cultures the overall decrease in the number of nodules was the main effect in N-starved cultures, whereas a delay in nodulation was more responsible for a loss in efficiency of N-sufficient cultures. Competition for nodulation was studied with young cultures of two wild-type strains differing only in their antibiotic resistance, the N-starved cultures being the most competitive.

Stimulation of adhesiveness, infectivity, and competitiveness for nodulation of Bradyrhizobium japonicum by its pretreatment with soybean seed lectin.

Soybean seed lectin stimulates adsorption of Bradyrhizobium japonicum to its host roots. Pretreatment of the rhizobia with soybean seed lectin for at least 6-12 h previous to their interaction with the plants was required to detect the stimulatory effect. This activity could be observed with as few as 1000 soybean seed lectin molecules per bacterium, and required specific carbohydrate binding. Infectivity and competitiveness for nodulation were also stimulated by preincubation of the rhizobia either with soybean seed meal extract or soybean seed lectin, the extract being more effective in enhancing competitiveness.