RESUMO
Respiratory virus infections are a major health concern and represent the primary cause of testing consultation and hospitalization for young children. The application of nucleic acid amplification technology, particularly multiplex PCR coupled with fluidic or fixed microarrays, provides an important new approach for the detection of multiple respiratory viruses in a single test. The aim of this study was to analyze respiratory samples from children with acute respiratory tract infection (ARTI) using a commercial array-based method (CLART(®) PneumoVir Genomica, Coslada, Spain). These tests were used to identify viruses in 281 nasopharyngeal samples obtained from children affected by ARTI. Samples were obtained form October 2008 to April 2009. Viruses were identified in 80% of the studied ARTI providing useful information on clinical features and epidemiology of specific agents affecting children in cold months. Multiple viral infections were found in 33.45% of the specimens.
Assuntos
Vírus de DNA/isolamento & purificação , Vírus de RNA/isolamento & purificação , Infecções Respiratórias/virologia , Viroses/virologia , Doença Aguda , Adolescente , Criança , Pré-Escolar , Feminino , Genótipo , Humanos , Técnicas Imunoenzimáticas , Lactente , Masculino , Análise em Microsséries , Nasofaringe/virologia , Reação em Cadeia da Polimerase/métodosRESUMO
Las infecciones por virus respiratorios representan la primera causa de consulta y hospitalización en la población pediátrica. El empleo de técnicas moleculares, principalmente aquellas basadas en PCR múltiple acoplada a detección por microarrays, supone un importante avance para la detección de varios de estos virus en un único ensayo. El objetivo de este estudio ha sido el análisis de muestras respiratorias procedentes de niños con infección respiratoria aguda (ARTI) mediante un método comercial (CLART® PneumoVir). Este método se basa en la amplificación y detección por microarrays de los 17 virus humanos más frecuentes en este tipo de patología. El ensayo se ha llevado a cabo en 281 muestras nasofaríngeas provinientes de niños con ARTI que acudieron al Hospital Clínico San Carlos de Madrid entre Octubre del 2008 y Abril del 2009. El 80% de las muestras estudiadas presentaron un resultado positivo para, al menos, uno de los 17 virus analizados proporcionando una valiosa información sobre las características clínicas y epidemiológicas de los agentes específicos que afectan a la población pediátrica en los meses fríos. Gracias a la técnica empleada pudieron detectarse infecciones múltiples en el 33,45% de las muestras(AU)
Respiratory virus infections are a major health concern and represent the primary cause of testing consultation and hospitalization for young children. The application of nucleic acid amplification technology, particularly multiplex PCR coupled with fluidic or fixed microarrays, provides an important new approach for the detection of multiple respiratory viruses in a single test. The aim of this study was to analyze respiratory samples from children with acute respiratory tract infection (ARTI) using a commercial array-based method (CLART® PneumoVir Genomica, Coslada, Spain). These tests were used to identify viruses in 281 nasopharyngeal samples obtained from children affected by ARTI. Samples were obtained form October 2008 to April 2009. Viruses were identified in 80% of the studied ARTI providing useful information on clinical features and epidemiology of specific agents affecting children in cold months. Multiple viral infections were found in 33.45% of the specimens(AU)
