RESUMO
BACKGROUND: Apoptosis, also known as programmed cell death, has been reported not only as a pathogenic mechanism, but also as a mechanism of resistance and control of a variety of infections. Particularly during HIV-1 infection, apoptosis is the main mechanism by which infected and uninfected CD4+ lymphocytes are eliminated. However, apoptosis as a mechanism of natural resistance to HIV infection has this far not been explored. OBJECTIVE: To determine whether apoptosis could explain, at least in part, the natural resistance to HIV infection observed in some exposed but uninfected individuals (ESN). RESULTS: Our data shows that peripheral blood monocytes in the ESN group has a predisposition to undergo spontaneous apoptosis, as well as apoptosis induced by HIV infection in vitro, compared with monocyte population from the control group at low risk of HIV infection. CONCLUSIONS: These findings suggest that, in some ESN individuals, monocytes could play an important role in the control of HIV infection by undergoing apoptosis. However, since the variability among individuals is large, studies with larger cohorts focusing in monocyte apoptosis as pathogenic mechanisms are required.
Assuntos
Apoptose , Infecções por HIV/imunologia , HIV-1 , Imunidade Inata , Adulto , Células Cultivadas , Feminino , Citometria de Fluxo , Infecções por HIV/sangue , Soronegatividade para HIV , HIV-1/patogenicidade , Humanos , Leucócitos Mononucleares/imunologia , Masculino , Pessoa de Meia-Idade , MonócitosRESUMO
1. The effect of chloride on serum angiotensin I-converting enzyme (ACE) activity was characterized in eight mammalian species: dog, guinea pig, hamster, human, mouse, rabbit, rat, and sheep. 2. Optimum chloride concentrations varied from 300 mM for rabbit to 1700 mM for hamster. 3. The increments with these optimum concentrations with respect to 100 mM chloride concentration were from 1.4-fold in rabbit to 7.9-fold in hamster and dog. 4. There was no correlation between serum chloride concentration or serum ACE activity and optimum chloride concentration. 5. Serum ACE increased only in humans with diamide pretreatment suggesting the presence of endogenous inhibitors.
