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1.
J Microsc ; 267(3): 371-383, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28665484

RESUMO

We report here a new microscopic technique for imaging and identifying sedimentary organic matter in geologic materials that combines inverted fluorescence microscopy with scanning electron microscopy and allows for sequential imaging of the same region of interest without transferring the sample between instruments. This integrated correlative light and electron microscopy technique is demonstrated with observations from an immature lacustrine oil shale from the Eocene Green River Mahogany Zone and mid-oil window paralic shale from the Upper Cretaceous Tuscaloosa Group. This technique has the potential to allow for identification and characterization of organic matter in shale hydrocarbon reservoirs that is not possible using either light or electron microscopy alone, and may be applied to understanding the organic matter type and thermal regime in which organic nanoporosity forms, thereby reducing uncertainty in the estimation of undiscovered hydrocarbon resources.

2.
ACS Nano ; 10(1): 265-73, 2016 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-26580231

RESUMO

We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.


Assuntos
Células Imobilizadas/ultraestrutura , Fibroblastos/ultraestrutura , Microscopia Eletrônica de Varredura/métodos , Imagem Óptica/métodos , Animais , Reatores Biológicos , Linhagem Celular , Células Imobilizadas/metabolismo , Chlorocebus aethiops , Endocitose/fisiologia , Fibroblastos/metabolismo , Microscopia Eletrônica de Varredura/instrumentação , Imagem Óptica/instrumentação , Pontos Quânticos/química , Pontos Quânticos/metabolismo
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