Assuntos
Glândula Parótida/patologia , Sialadenite/diagnóstico , Tuberculose Bucal/diagnóstico , Adulto , Antituberculosos/uso terapêutico , DNA Bacteriano , Diagnóstico Diferencial , Feminino , Humanos , Glândula Parótida/microbiologia , Reação em Cadeia da Polimerase , Sialadenite/genética , Sialadenite/patologia , Tuberculose Bucal/genética , Tuberculose Bucal/patologiaRESUMO
We present two cases of biopsy proven tuberculosis of the pancreas in non-immunocompromised patients diagnosed and treated in our unit within the last 14 years. The first case presented with abdominal pain and fever, and the second with iron deficiency anaemia and severe weight loss. In both cases abdominal ultrasound and computed tomography suggested a pancreatic carcinoma. There was no pulmonary or intestinal tuberculosis. The tuberculin skin test was positive. Upon exploratory laparotomy the macroscopic appearance of the pancreas was that of an inoperable pancreatic carcinoma. Following the histological diagnosis of pancreatic tuberculosis, both patients were successfully treated with triple antituberculous therapy for 6 months. Isolated pancreatic tuberculosis is an extremely rare disease with only 41 cases in non-immunocompromised patients reported worldwide (1966-1997). It is a curable disease and should be considered in the differential diagnosis of a pancreatic mass or abscess shown on ultrasound or computed tomography, especially in developing countries, where tuberculosis is common.
Assuntos
Pancreatopatias , Tuberculose , Idoso , Antituberculosos/uso terapêutico , Feminino , Humanos , Pessoa de Meia-Idade , Pancreatopatias/diagnóstico , Pancreatopatias/tratamento farmacológico , Pancreatopatias/patologia , Tomografia Computadorizada por Raios X , Tuberculose/diagnóstico , Tuberculose/tratamento farmacológico , Tuberculose/patologiaRESUMO
The proteolytic degradation of labelled pyromycyl polypeptides was investigated in human intact erythroid cells derived from the bone marrow of eight non-thalassaemic patients and the peripheral blood of eleven thalassaemics (eight splenectomized beta thalassaemia heterozygotes and three sickle-cell beta thalassaemics). These abnormal polypeptides are rapidly degraded to soluble trichloroacetic-acid (TCA) fragments with a half-life of 12 min both in bone marrow and peripheral blood. This comes very close to the half-life reported for the puromycyl peptide degradative system in rabbit reticulocytes (15 min). The relationship of the present proteolytic system to the ATP-dependent one, described in rabbit reticulocytes, and to that responsible for the free alpha-chain degradation in beta thalassaemia is discussed.
Assuntos
Eritroblastos/metabolismo , Peptídeos/metabolismo , Puromicina/metabolismo , Talassemia/patologia , Células da Medula Óssea , Meia-Vida , Humanos , Hidrólise , Talassemia/sangue , TrítioRESUMO
Globin chain synthesis was studied in reticulocytes and bone marrow erythroid precursors in four sickle beta-thalassaemic Greek patients. Significant globin chain imbalance was found in reticulocytes (alpha/gamma + beta A + beta S = 2.20 +/- SD 0.16) and bone marrow (alpha/gamma + beta A + beta S = 1.58 +/- SD 0.11) after two hours' incubation. There was evidence of contamination of the gamma, beta A, and, to a lesser extent, of the beta S chain by non-haem proteins. The contamination was more obvious in chromatograms obtained from whole cell bone marrow samples and could partially explain the lower alpha/non-alpha ratio found in bone marrow.