RESUMO
The aim of this study was to investigate the distribution of different morphological features in different layers of lyophilized adipose tissue. In this work the scanning electron microscopy (SEM) was adopted for investigation of lyophilized adipose tissue taken from obese patients. The adipose tissue was taken from subcutaneous (SAT), preperitoneal (PAT) and visceral (VAT) layers of adipose tissue. The obtained results of the main microstructural features provided information about morphological features of subcutaneous, preperitoneal and visceral layers in obese people. The obtained SEM results possibly could be used for the estimation of metabolic symptoms and prediction different diseases. The SEM method was never used before to investigate morphology of SAT, PAT and VAT layers of lyophilized human adipose tissue.
Assuntos
Tecido Adiposo/ultraestrutura , Liofilização , Gordura Intra-Abdominal/fisiopatologia , Síndrome Metabólica/fisiopatologia , Obesidade/fisiopatologia , Gordura Subcutânea/fisiopatologia , Feminino , Humanos , Gordura Intra-Abdominal/ultraestrutura , Masculino , Síndrome Metabólica/complicações , Síndrome Metabólica/diagnóstico , Microscopia Eletrônica de Varredura , Obesidade/complicações , Obesidade/diagnóstico , Gordura Subcutânea/ultraestruturaRESUMO
Reduction of Cd(II) on a dropping mercury electrode was used to study interaction of beta-cyclodextrin with Cd(II) ions. It was found that Cd(II) forms Cdbeta-CD(OH)(2)(2-) hydroxy-complex with the anion of beta-cyclodextrin in alkaline solutions (pH>11), the logarithm of stability constant being 10.4+/-0.1 (20 degrees C; I=1.0). The calculated value of the diffusion coefficient equal to 1.0x10(-6)cm(2)/s shows a large size Cd(II) complex species formation in alkaline solutions containing beta-CD.
Assuntos
Cádmio/química , Ciclodextrinas/química , beta-Ciclodextrinas , Cádmio/metabolismo , Ciclodextrinas/metabolismo , Difusão , Polarografia/métodos , SoluçõesRESUMO
Polarographic and UV-spectrophotometric investigations of Pb(II) complex formation with beta-cyclodextrin have showed that the complexation of Pb(II) ions begins at pH >10. The formation of lead(II) 1:1 complex with the beta-cyclodextrin anion was observed at pH 10-11.5. The logarithm of the stability constant of this complex compound is 15.9+/-0.3 (20 degrees C, ionic strength 1.0), and the molar extinction coefficient value is ca. 5500 (lambda(max)=260 nm). With further increase in solution pH the Pb-beta-cyclodextrin complex decomposes and converts to Pb(OH)(2) or Pb(OH)(3)(-) hydroxy-complexes. This process occurs with a decrease in Pb(II) complexation degree. The latter result could be explained by a decrease in the beta-cyclodextrin anion activity. Neither Pb(OH)(2) nor Pb(OH)(3)(-) encapsulation into beta-CD cavity was observed.
