Effects of Succinate-Containing Preparation on Characteristics of Rat Erythrocytes in Exhaustive Swimming with a Load.

We studied the dynamics of the red blood cell composition of Wistar male rats at rest and when swimming with a load (4% body weight) before and after administration of a succinatecontaining preparation (meso-2,3-dimercaptosuccinic acid). In rats receiving the succinatecontaining preparation, a decrease in the number of red blood cells and an increase in their volume and absolute and relative number of reticulocytes were observed at rest in comparison with vivarium control. In rats exposed to exhaustive swimming after preliminary administration of the test preparation (12 h before the test), we observed a decrease in hematocrit and erythrocyte diameter in comparison with the corresponding parameters in rats not treated with the preparation; the level of hemoglobin did not change. The pattern of changes in the cellular composition of red blood in rats at rest and during swimming against the background of treatment with the succinate-containing preparation in comparison with vivarium control is considered as a result of its effect on physical exercise under conditions of stabilization of hemoglobin and hematocrit levels, activation of proliferative activity of red bone marrow, and an increase in time of swimming to exhaustion by 2.8 times.

[Serum glucose level in acute severe hypoxia in human at rest].

Study of the effects of acute normobaric hypoxia was performed on a group of young healthy males (age 19-23, n = 10). Conditions of acute normobaric hypoxia were modeled by using oxygen-nitrogen mixture containing 8% of oxygen as a breath gas. That level of oxygen corresponds with its partial pressure at 7000 meters above sea level. A number of different analyses were conducted during experiment: levels of glucose, pyruvate, lactate in peripheral venous blood, hemoglobin, pH, hematocrit, partial pressures of 02 and CO2, hemoglobin saturation. It was shown that on 5th minute of hypoxia serum glucose level decreased significantly (p < 0.05). The average decrease was about 0.76 mmol/l in its maximum and lowest individual glucose levels were 4.0 mmol/l or higher. Serum glucose level returned to background values (assessed before the test) at 10th minute of experiment. In our opinion results of the study suggest that syncopal form of height hypoxia may be caused by the reasons other than hypoglycemia.

[Fatty acid of human plasma under the influence of acute strong normobaric hypoxia].

The influence of acute normobaric hypoxia on total pool of human plasma fatty acids was studied in experiment (respiration of hypoxic gas mixture containing 8% of O2, during 25 min). Health status of participants-volunteers and the hypoxia intensity were monitored with a complex of electrophysiological methods: EEC, ECG, measurement of blood pressure, SpO2, evaluation of cardiac output, gas analysis of exhaled air. Using gas chromatography we studied plasma fatty acids total profile (esterificated and non-esterificated) before hypoxia exposure, on 2nd, 5th, 10th, 20th minutes of acute hypoxia and on 5th and 15th minutes of recovery. It was shown that blood saturation value dropped to 52.1 +/- 7.1 % on 25th minute of hypoxia exposure. There were no statistically significant changes in concentrations of total fatty acids, saturated fatty acids, monounsaturated palmitoleic acid, polyunsaturated linoleic and linolenic acids. Significant (p < 0.05) decrease of concentration of oleic and arachidonic acids was displayed that persisted after 15 minutes of recovery period. It is possible to assume that these changes in unsaturated fatty acid concentration were caused by activation of free radical oxidation processes and production of oleic acid derivatives. The performed hypoxic test seemed to be sufficient in order to reveal hypoxia effects on metabolism of individual unsaturated fatty acids.

[Seasonal aspects of the oxidative stress in Northeners].

Evaluated were consequences of changes in oxygen demand, bronchial conduction, exercise performance, functioning of antioxidant enzymes and body provision with natural oxidants for the level of free-radical oxidation products in human organism. To this end, a group of essentially healthy young male northerners spending much time outdoors was surveyed over a one-year period. Among the listed parameters oxidation of free radicals was particularly sensitive to seasonal insufficiency of some of the antioxidant enzymes.