[The dependence of blood and tissue proteolytic activity during acute stress from A type of the organism reaction]. / Zalezhnist' proteolitychnoï aktyvnosti krovi i tkanyn pry hostromu stresi vid typu reahuvannia tvaryn.

In the experiments on rats there was grounded the dependence of proteolytic processes in tissues during acute emotional painful stress from typological peculiarities of the organism that was defined by the method of "Open field".

[Effect of contrical on proteolytic enzymes of blood serum in acute emotional-pain stress]. / Vliianie kontrikala na aktivnost' proteoliticheskikh fermentov syvorotki krovi pri ostrom émotsional'no-bolevom stresse.

General proteolytic and antitryptic blood serum activity in acute emotionally-painful stress was investigated as well as that on the background of the before injected contrical. It was determined that in case of a stress there increases the general proteolytic activity of blood serum and at the same time antiproteolytic activity decreases. The coefficient of the relation of antiproteolytic activity to proteolytic activity of blood serum in case of a stress may become of 50% of the control. The introduction of inhibitor of proteinase contrical before the beginning of stressor influence promotes the decrease of general proteolytic activity to the level of the control indexes.

[Prospects for using DNA probes for rapid diagnosis of antibiotic resistance in clinical strains of enteric bacteria]. / Perspektivy ispol'zovaniia DNK-zondov dlia ékspress-diagnostiki antibiotikorezistentnosti klinicheskikh shtammov énterobakterii.

Aminoglucoside resistance patterns of clinical strains of enteric bacteria isolated from inpatients of Moscow clinics were determined. APH(3')-I and AAC(3)-II were shown to be the most frequent. The aphA1 and aacC2 genes encoding the enzymes were cloned from the R plasmid of the transconjugant of the E. coli clinical strains. DNA probes based on the determined nucleotide sequences of the cloned genes were constructed and used in DNA-DNA hybridization experiments. The results on the occurrence of APH(3')-I and AAC(3)-II in the strains tested were confirmed by the DNA-DNA hybridization. Prospects for developing a set of DNA probes for rapid diagnosis of antibiotic resistance are discussed.