Assuntos
Infecções por Campylobacter/microbiologia , Campylobacter fetus/isolamento & purificação , Diarreia Infantil/microbiologia , Fezes/microbiologia , Criança , Pré-Escolar , Diarreia Infantil/etiologia , Enterobacteriaceae/isolamento & purificação , Infecções por Enterobacteriaceae/microbiologia , Humanos , Lactente , Recém-Nascido , Testes de Sensibilidade MicrobianaRESUMO
Se estudiaron 2.690 muestras provenientes de ninos enfermos para el aislamiento de Campylobacter fetus subsp. jejuni y otros patogenos entericos. Se identificaron 18 cepas de Campylobacter fetus subsp. jejuni lo que se correspondio con el 6,38% del total de patogenos identificados y con el 0,67% del total de casos estudiados.Las 18 cepas estudiadas fueron en un 100% resistentes a la penicilina, ampicillin, novobiocina, metacillin, oxacillin y colymicin mientras que todos fueron sensibles a la eritromicina, estreptomicina y acido nalidixico. Se correspondio con los meses de invierno el mayor por ciento de aislamiento. En ninguno de los casos estudiados coincidio el Campylobacter fetus subsp.jejuni con otra bacteria enterica patogena Entre los otros patogenos entericos buscados en estas muestras hubo predominio de la Escherichia coli enteropatogena. No se aislaron Yersinia enterocolitica ni Escherichia coli enteroinvasivo
Assuntos
Humanos , Campylobacter fetus , Meios de Cultura , FezesRESUMO
Nineteen Campylobacter jejuni strains survived best in suspensions with approximately 10(9) bacteria in 4% albumin solution at-60 degrees C for 17 months (18 out of 19). At the same temperature and during the same period they survived less well in milk (11 out of 19). Recovery of campylobacters after 7 weeks was abundant in albumin, and somewhat less in milk. At -20 degrees C survival in milk was worse; 17 out of 19 cultures did not survive 5 weeks in milk and 7 died in albumin. At 4 degrees C all but one strain survived two weeks in milk and none died in albumin, but after 3 weeks only 7 survived in milk and 2 in albumin. At room temperature campylobacters did not survive 10 days in any of the media used. Peptone water (4%) and peptone-saline proved unsuitable for preservation. From 20 positive stool samples kept at 4 degrees C, on the day after the first sampling C. jejuni could not be recovered from 10 samples; bacteria survived for 2-7 days in 8 samples, for 12-20 days in 2 samples.
