Impact of using glucose as a sole carbon source to analyze the effect of biochar on the kinetics of biomethane production.

The adaptation of biochar in anaerobic digestion (AD) positively influences the conversion of substrate to biomethane and promotes system stability. This study investigated the influence of biochar (BC) doses (0 to 8 g/L) on the Biochemical Methane Potential (BMP) of glucose during a 60-day AD in a mesophilic batch-type reactor. The first 6.5 weeks of the experimentation were dedicated to the microorganism's adaptation to the biochar and degradation of organics from the used inoculum (3 phases of the glucose feeding). The last 2 weeks (4th phase of glucose feeding) represented the assumption, that glucose is the sole carbon source in the system. A machine learning model based on the autoregressive integrated moving average (ARIMA) method was used to model the cumulative BMP. The results showed that the BMP increased with the amount of BC added. The highest BMP was obtained at a dose of 8 g/L, with a maximum cumulative BMP of 390.33 mL CH4/g-VS added. Likewise, the system showed stability in the pH (7.17 to 8.17). In contrast, non-amended reactors produced only 135.06 mL CH4/g-VS and became acidic at the end of the operation. Reducing the influence of carbon from inoculum, sharpened the positive effect of BC on the kinetics of biomethane production from glucose.

Direct interspecies electron transfer mechanisms of a biochar-amended anaerobic digestion: a review.

This paper explores the mechanisms of biochar that facilitate direct interspecies electron transfer (DIET) among syntrophic microorganisms leading to improved anaerobic digestion. Properties such as specific surface area (SSA), cation exchange capacity (CEC), presence of functional groups (FG), and electrical conductivity (EC) were found favorable for increased methane production, reduction of lag phase, and adsorption of inhibitors. It is revealed that these properties can be modified and are greatly affected by the synthesizing temperature, biomass types, and residence time. Additionally, suitable biochar concentration has to be observed since dosage beyond the optimal range can create inhibitions. High organic loading rate (OLR), pH shocks, quick accumulation and relatively low degradation of VFAs, and the presence of heavy metals and toxins are the major inhibitors identified. Summaries of microbial community analysis show fermentative bacteria and methanogens that are known to participate in DIET. These are Methanosaeta, Methanobacterium, Methanospirillum, and Methanosarcina for the archaeal community; whereas, Firmicutes, Proteobacteria, Synergistetes, Spirochetes, and Bacteroidetes are relatively for bacterial analyses. However, the number of defined cocultures promoting DIET is very limited, and there is still a large percentage of unknown bacteria that are believed to support DIET. Moreover, the instantaneous growth of participating microorganisms has to be validated throughout the process.

Influence of Pre-Incubation of Inoculum with Biochar on Anaerobic Digestion Performance.

The application of biochar as an additive to enhance the anaerobic digestion (AD) of biomass has been extensively studied from various perspectives. This study reported, for the first time, the influence of biochar incubation in the inoculum on the anaerobic fermentation of glucose in a batch-type reactor over 20 days. Three groups of inoculum with the same characteristics were pre-mixed once with biochar for different durations: 21 days (D21), 10 days (D10), and 0 days (D0). The BC was mixed in the inoculum at a concentration of 8.0 g/L. The proportion of the inoculum and substrate was adjusted to an inoculum-to-substrate ratio of 2.0 based on the volatile solids. The results of the experiment revealed that D21 had the highest cumulative methane yield, of 348.98 mL, compared to 322.66, 290.05, and 25.15 mL obtained from D10, D0, and the control, respectively. Three models-modified Gompertz, first-order, and Autoregressive Integrated Moving Average (ARIMA)-were used to interpret the biomethane production. All models showed promising fitting of the cumulative biomethane production, as indicated by high R2 and low RMSE values. Among these models, the ARIMA model exhibited the closest fit to the actual data. The biomethane production rate, derived from the modified Gompertz Model, increased as the incubation period increased, with D21 yielding the highest rate of 31.13 mL/gVS. This study suggests that the application of biochar in the anaerobic fermentation of glucose, particularly considering the short incubation period, holds significant potential for improving the overall performance of anaerobic digestion.

Investigation of the performance of a cylindrical hopper and metering device of a carrot seeder.

A carrot seeder that utilizes a cylindrical component serving as both hopper/metering device to deposit seeds precisely into planting beds/hills at a uniform interval along a straight row over a plant bed was developed in this study. The seeder was evaluated at different operating speeds (89, 70, 61, 51, 48, 38, and 34 cm s-1) relative to mean number of seeds planted in each hill, hill center, scattering distance ratio, hill spacing and missed hills. The seeder was able to deposit seeds in each hill ranging from 2.8 to 4.0 at all speeds. The mean hill center and missed hills suggested that the seeder is best operated at speed under 70 cm s-1 with the respective values ranging from 0.9 to 1.6 cm and 0-5.5%. This indicates that number of the seeds dropped per hill was very uniform, which is a good indicator of the seeding performance. The hill center and missed hills at 89 cm s-1 were 3.08 cm and 16.67% respectively were significantly higher than at lower speeds. Other operating performances such as the mean number of seeds deposited in each hill and the distance between hills did not appear to vary with the planting speed. The scattering distance ratios of operating speeds from 34 to 61 cm s-1 were less than 30% and therefore acceptable for hill dropping of carrot seeds.