Assuntos
Transformação Celular Neoplásica , Neoplasias Experimentais/etiologia , Papillomaviridae , Polyomaviridae , Adenocarcinoma/etiologia , Animais , Linhagem Celular , Cricetinae , Feminino , Mesocricetus , Transplante de Neoplasias , Neoplasias Experimentais/patologia , Papillomaviridae/isolamento & purificação , Sarcoma Experimental/etiologia , Transplante HomólogoRESUMO
Simian papovavirus SA12 agglutinated human, guinea pig, and chicken erythrocytes. SA12 hemagglutinin was most effectively released from debris of infected tissue culture cells at an alkaline pH.
Assuntos
Hemaglutinação por Vírus , Polyomavirus/imunologia , Animais , Formação de Anticorpos , Vírus BK/imunologia , Reações Cruzadas , Haplorrinos , Concentração de Íons de Hidrogênio , Vírus 40 dos Símios/imunologiaRESUMO
SA12 virus, originally isolated from an uninoculated South African vervet monkey kidney culture, was identified as a new member of the simian virus 40 (SV40)-polyoma subgroup of papovaviruses. The virus produced a cytopathic effect with nuclear enlargement in primary rhesus kidney cells. The virion had papovavirus morphology and a diameter of 44 to 45 nm. The DNA of the virus was a circular, double-stranded, superhelical molecule with a mean length 101% that of SV40 DNA and an estimated molecular weight of 3.3 X 10(6). The virus was found to be unrelated to other papovaviruses by neutralization, immune electron microscopy, and immunofluorescence tests with antiviral sera. SA12 virus-infected cells exhibited a capsid antigen, which has recently been found to be common to viruses of the SV40-polyoma subgroup. The virus readily transformed kideny cells from 10-day-old hamsters. Inoculation of transformed cells produced tumors in 3- to 4-week-old hamsters. The T antigens of SA12 and SV40 viruses were strongly and reciprocally cross-reactive. A high proportion of the sera of chacma baboons, Papio ursinus, and a comparatively lower proportion of the sera of vervet monkeys, Cercopithecus pygerythrus, had neutralizing antibodies to SA12 virus