RESUMO
This retrospective study reports on the survival of two-piece angulated prosthetic platform (APP) implants consecutively placed at a specialist periodontics clinic with a mean follow-up of 28.2 ± 15.6 months (range 4.0-71.0). For 183 recalled patients that received 239 implants, modelled survival at follow-up up to 71 months was 99.2% and 91.1% at the patient and implant level respectively. The majority of patients were non-smokers and non-diabetics, with a quarter having a history of treated periodontitis. Eighty percent of patients received a single APP implant. Sixty-three percent of implants supported a single crown, 28% a fixed partial denture, and 9% a fixed complete denture. Nearly all implants were placed either at the time of tooth extraction or after complete bone healing, in approximately equal numbers. Three quarters had adjunct bone grafting with a quarter having adjunct soft tissue grafting. For 210 surviving and restored implants with satisfactory intra-oral radiographs taken at last recall with a mean follow-up 28.4 ± 15.5 months (range 4.0-71.0), the mean radiographic bone levels were -0.70 ± 0.87 mm (range -3.60 - +2.15). The results demonstrated clinically successful use of this unique geometry implant for multiple applications with acceptable short to medium term clinical outcomes.
RESUMO
BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers and the fourth leading cause of cancer-related deaths worldwide. We aimed to determine the role of miR-650 in CRC pathogenesis. METHODS: In this study, we examined the expression of miR-650 and KISS1 in 80 CRC patients who either received or did not receive chemo agents. For this aim, we assessed the miR-650 and KISS1 expression levels in 80 CRC tissues, 30 of which had no history of chemotherapy. The effect of miR-650 and 5-FU on KISS1 expression was measured using qPCR and Western blotting. Also, the 5- FU effect on miR-650 expression in the CRC cell lines was measured by qRT-PCR. Next, MTT assay and Flowcytometry assays were conducted to determine the role of miR-650 in cell viability and apoptosis. RESULTS: The results showed that miR-650 was down-regulated in CRC tissues. However, patients who received 5-FU before surgery showed increased expression of miR-650. The results for KISS1 were insignificant while administering 5-FU to patients preoperatively increased its expression. In-vitro studies showed that 5-FU led to the up-regulation of miR-650 in the SW480 CRC cell line. Furthermore, the administration of miR-650 and 5-FU downregulated KISS1, especially when combined. Moreover, miR-650 with 5-FU significantly reduced cell viability in CRC cell lines by inducing apoptosis. CONCLUSIONS: These results indicate that miR-650 has a tumor suppressive function, overcoming 5-FU chemoresistance in CRC, and induces apoptosis probably by alleviating KISS1. These results suggest that miR-650 is a potential contributor to CRC pathogenesis.
Assuntos
Neoplasias Colorretais , MicroRNAs , Humanos , Regulação para Baixo/genética , MicroRNAs/metabolismo , Fluoruracila/farmacologia , Fluoruracila/uso terapêutico , Kisspeptinas/genética , Linhagem Celular Tumoral , Apoptose/genética , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Resistencia a Medicamentos Antineoplásicos/genética , Regulação Neoplásica da Expressão Gênica/genética , Proliferação de Células/genéticaRESUMO
Colorectal cancer is the main cause of human death due to cancer. This fact could be due to the insufficiency of early diagnosis or poor therapeutic strategies. Various molecular tools have been utilized in studies to assess their potentials as diagnostic biomarkers or determining factors in precision medicine. Among these molecules, long non-coding RNAs (lncRNA) have been emerging as accurate and potent transcripts to improve the detection of cancer. The overexpressed lncRNAs could also be deeply studied as the molecules for the targeted therapy in different malignancies, in particular colorectal cancer. Thus, we utilized an unbiased approach to select the up-regulated lncRNAs in colon adenocarcinoma via analyzing the TCGA dataset. Then, we validated the overexpression of two first-ranked lncRNAs, i.e., NPSR1-AS1 and TLX1NB, in our in-house colorectal cancer samples as compared to the paired adjacent normal tissues. The analyses revealed that these lncRNAs could significantly distinguish the tumor against the normal samples. The results may have implications in the early diagnosis and targeted therapy of colorectal cancer.
