RESUMO
A bacterium with the ability to produce appreciable amount of exopolysaccharide was isolated from slimy layer of coconut. 16S rDNA analysis identified the organism as Bacillus circulans. EPS production was observed at all stages of culture growth and reached maximum of 0.065mg/ml by 96h, which on further incubation started to decrease. Response Surface Methodology using Box Behnken design has shown the influence of sucrose which was found to be directly proportional to exopolysaccharide production with production reaching 1.09mg/ml. HPLC analysis identified the presence of glucose, mannose, fructose and verbascose and NMR analysis confirmed the presence of glucose, mannose and galactose. Even though the extracted B. circulans EPS did not show appreciable anti-bacterial or anti-fungal activity, it exhibited appreciable antioxidant, anti-inflammatory and anti-tumor activity.
Assuntos
Bacillus/metabolismo , Polissacarídeos Bacterianos/biossíntese , Polissacarídeos Bacterianos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/metabolismo , Anti-Inflamatórios não Esteroides/farmacologia , Antineoplásicos/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Antioxidantes/química , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Chlorocebus aethiops , Células Hep G2 , Humanos , Modelos Teóricos , Polissacarídeos Bacterianos/química , Células VeroRESUMO
The bacterium with dye degrading ability was isolated from effluent disposal sites of textile industries, Tirupur and was identified as Moraxella osloensis based on the biochemical and morphological characterization as well as 16S rRNA sequencing. This organism was found to decolorize 87 % of Mordant Black 17 at 100 mg l⻹ under shake culture condition compared to 92 % under stationary culture condition. Maximum degradation of the dye by M. osloensis was achieved when the mineral salt medium was supplemented with 0.5 % glucose and 0.1 % ammonium nitrate at 35 °C. Degradation of dye was found to follow first order kinetics with the k value of 0.06282 h⻹ and a R² value of 0.955. Analyses for the identification of intermediate compounds confirmed the presence of naphthalene, naphthol, naphthoquinone, salicylic acid and catechol. Based on this finding a probable pathway for the degradation of Mordant Black 17 by M. osloensis has been proposed.
Assuntos
Compostos Azo/metabolismo , Poluentes Ambientais/metabolismo , Moraxella/metabolismo , Biotransformação , Meios de Cultura/química , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Microbiologia Ambiental , Resíduos Industriais , Redes e Vias Metabólicas , Dados de Sequência Molecular , Moraxella/isolamento & purificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
Pseudomonas aeruginosa CLRI BL22 was able to degrade metanilic acid, a sulfonated aromatic amine upto a concentration of 1,500 mg l(-1). The degradation of metanilic acid started when the organism reached its exponential growth phase. Chromatographic and spectrophotometric analyses confirmed the presence of aniline and ß-keto adipic acid indicating the ortho pathway reaction. Further proof for this pathway was provided by dioxygenase activity of the strain grown in the medium containing metanilic acid and glucose.
RESUMO
Pseudomonas aeruginosa, isolated from soil near tannery effluent was able to degrade 8-anilino-1-naphthalenesulfonic acid (ANSA), a sulfonated aromatic amine. The organism degraded this amine up to a concentration of 1,200 mg l(-1) using glucose and ammonium nitrate as carbon and nitrogen sources respectively. The degradation started when the organism reached its late exponential growth phase. Salicylic acid and beta-ketoadipic acid were identified as intermediate compounds using HPLC and GC-MS and provide evidence for ortho pathway reactions. Further proof for the pathway is obtained from the dioxygenase activity of the strain growing exponentially in medium with ANSA and glucose.
Assuntos
Naftalenossulfonato de Anilina/metabolismo , Proteínas de Bactérias/metabolismo , Dioxigenases/metabolismo , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/crescimento & desenvolvimento , Adipatos/análise , Proteínas de Bactérias/análise , Biodegradação Ambiental , Cromatografia Líquida de Alta Pressão , Dioxigenases/análise , Cromatografia Gasosa-Espectrometria de Massas , Pseudomonas aeruginosa/isolamento & purificação , Ácido Salicílico/análiseRESUMO
The mechanism by which Pseudomonas aeruginosa degraded Navitan Fast Blue S5R was studied using TLC, FTIR, HPLC and GC-MS analysis. Degradation started with the reduction of azo bonds producing metanilic acid and peri acid whose presence was confirmed by HPLC. Aniline, the desulfonated product from metanilic acid and salicylic acid could also be detected by HPLC. GC-MS analysis of the degradation products confirmed the presence of aniline and revealed the presence of beta-ketoadipic acid. Based on these products a probable pathway has been proposed for the degradation of Navitan Fast Blue S5R by Pseudomonas aeruginosa.
