RESUMO
The LIM and SH3 protein 1 (LASP1) is a focal adhesion protein. Its expression is increased in many malignant tumors. However, little is known about the physiological role of the protein. In the present study, we investigated the expression and function of LASP1 in normal skin, melanocytic nevi and malignant melanoma. In normal skin, a distinct LASP1 expression is visible only in the basal epidermal layer while in nevi LASP1 protein is detected in all melanocytes. Melanoma exhibit no increase in LASP1 mRNA compared to normal skin. In melanocytes, the protein is bound to dynamin and mainly localized at late melanosomes along the edges and at the tips of the cell. Knockdown of LASP1 results in increased melanin concentration in the cells. Collectively, we identified LASP1 as a hitherto unknown protein in melanocytes and as novel partner of dynamin in the physiological process of membrane constriction and melanosome vesicle release.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proteínas do Citoesqueleto/metabolismo , Proteínas com Domínio LIM/metabolismo , Melaninas/metabolismo , Melanócitos/metabolismo , Melanoma/metabolismo , Nevo Pigmentado/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Adesão Celular , Movimento Celular , Proliferação de Células , Células Cultivadas , Proteínas do Citoesqueleto/genética , Dinaminas/metabolismo , Técnicas de Silenciamento de Genes , Humanos , Proteínas com Domínio LIM/genética , Melanoma/genética , Nevo Pigmentado/genéticaRESUMO
The present study was carried out to evaluate the effect of HAP-EVA, fibrin glue, HA-BG, Latex and Dental material on oxidative stress related mtDNA damage by in vitro and in vivo methods. In vivo studies of these biomaterials were carried out by implanting biomaterials (five materials) on animals for period of 1, 4, 12, 26 and 52 weeks. At the end of observations, animals were anesthetized, sacrificed and tissues surrounding the implanted materials were collected. Brain, bone and muscles were used for the extraction of mtDNA. Similarly mtDNA was extracted from the homogenate of fresh brain, bone and muscles on exposure to the physiological saline extract of all the above five biomaterials (In vitro). The extracted mtDNA were subjected to analyse the presence of 8-OHdG. The results of study indicated that there was no significant increase in the level of 8-OHdG and thereby does not influence on the GC-TA transversions.
