1.
Ned Tijdschr Geneeskd
; 142(18): 1043-4; author reply 1044, 1998 May 02.
Artigo
em Holandês
| MEDLINE
| ID: mdl-9623210
2.
Histochem J
; 17(9): 1043-52, 1985 Sep.
Artigo
em Inglês
| MEDLINE
| ID: mdl-3905722
RESUMO
A well-characterized monoclonal antibody against human lysosomal alpha-glucosidase has been used for the immunohistochemical localization of the enzyme in cultured human skin fibroblasts. Under conditions that are routinely used for the preparation of cells for immunocytochemistry, this monoclonal antibody does not react with acid alpha-glucosidase but in contrast with components of the cytoskeleton. Double-labelling experiments with the monoclonal antibody and rabbit anti-vimentin antiserum identified the cytoskeletal components as intermediate filaments. The implications of this observation for the use of monoclonal antibodies in immunocytochemistry in general are discussed.