RESUMO
A prospective study compared fecal isolation rates of Campylobacter concisus for children with diarrhea and without diarrhea by a filter technique in which media were incubated for 4 days in a microaerobic atmosphere. No statistically significant difference in isolation rates was found (13.2% in patients with diarrhea and 9% in controls). Moreover, 35 of 37 children attending the same day care center harbored different C. concisus strains, as was demonstrated by arbitrary primer PCR DNA fingerprinting. These data suggest a lack of a pathogenic role for C. concisus in enteritis.
Assuntos
Campylobacter/isolamento & purificação , Diarreia/microbiologia , Fezes/microbiologia , Campylobacter/genética , Pré-Escolar , Impressões Digitais de DNA , DNA Bacteriano/análise , Humanos , LactenteRESUMO
In the autumn of 1983, an outbreak of recurrent abdominal cramps occurred in a nursery and primary school in the Rovigo area in Italy. None of the 10 affected children had diarrhea. An atypical Campylobacter-like organism was isolated from feces in all cases. Conventional enteropathogens were searched for but not detected. The Campylobacter-like organism was identified as Arcobacter butzleri by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and cellular fatty acid analysis. Its identity was confirmed by DNA-DNA hybridizations versus Arcobacter reference strains. All of the preserved outbreak strains have identical protein profiles and phenotypic characteristics and belong to serogroup 1 of the Lior serotyping scheme on the basis of slide agglutination of crude and absorbed antisera of A. butzleri reference strains versus heat-labile antigens of live bacteria. These data point to an epidemiological relationship. The successive timing of the cases suggests person-to-person transmission.
Assuntos
Infecções por Campylobacter/microbiologia , Campylobacter/patogenicidade , Surtos de Doenças , Gastroenteropatias/microbiologia , Campylobacter/classificação , Campylobacter/isolamento & purificação , Infecções por Campylobacter/epidemiologia , Criança , Pré-Escolar , Fezes/microbiologia , Feminino , Humanos , Itália/epidemiologia , Masculino , Fenótipo , VirulênciaRESUMO
During an 18-month period all stools submitted to a microbiology laboratory in Belgium for culture were screened for Verocytotoxin-producing Escherichia coli (VTEC) serotype O157. In the stool samples from 3940 patients, eight (0.2%) VTEC O157 strains were isolated, seven of which were O157:H7. Additional screening for other serotypes of VTEC in 332 selected stool samples yielded four more strains (serotypes O2:K1:H6, O111:H-, O117:K1:H7 and O"C70/86":H-). The 0.3% isolation rate for all VTEC was comparable to that for Shigella spp. Eight children under 30 months and two adults suffered from uncomplicated gastroenteritis. A 5-month-old child and a 41-year-old woman presented with hemolytic uremic syndrome a few days after onset of a diarrheal episode.