Glass transition accelerates the spreading of polar solvents on a soluble polymer.

We study the wetting of polymer layers by polar solvents. As previously observed, when a droplet of solvent spreads, both its contact angle and velocity decrease with time as a result of solvent transfers from the droplet to the substrate. We show that, when the polymer is initially glassy, the angle decreases steeply for a given value of the velocity, Ug. We demonstrate that those variations result from a plasticization, i.e., a glass transition, undergone by the polymer layer during spreading, owing to the increase of its solvent content. By analyzing previous predictions on the wetting of rigid and soft viscoelastic substrates, we relate Ug to the viscosity of the polymer gel close to the glass transition. Finally, we derive an analytical prediction for Ug based on existing predictions for the water transfer from the droplet to the substrate. Using polar solvents of different natures, we show that the experimental data compare well to the predicted expression for Ug.

Dynamic study of blood-brain barrier closure after its disruption using ultrasound: a quantitative analysis.

Delivery of therapeutic or diagnostic agents to the brain is majorly hindered by the blood-brain barrier (BBB). Recently, many studies have demonstrated local and transient disruption of the BBB using low power ultrasound sonication combined with intravascular microbubbles. However, BBB opening and closure mechanisms are poorly understood, especially the maximum gap that may be safely generated between endothelial cells and the duration of opening of the BBB. Here, we studied BBB opening and closure under magnetic resonance (MR) guidance in a rat model. First, MR contrast agents (CA) of different hydrodynamic diameters (1 to 65 nm) were employed to estimate the largest molecular size permissible across the cerebral tissues. Second, to estimate the duration of the BBB opening, the CA were injected at various times post-BBB disruption (12 minutes to 24 hours). A T(1) mapping strategy was developed to assess CA concentration at the ultrasound (US) focal point. Based on our experimental data and BBB closure modeling, a calibration curve was obtained to compute the half closure time as a function of CA hydrodynamic diameter. These findings and the model provide an invaluable basis for optimal design and delivery of nanoparticles to the brain.

Low-gradient single-sided NMR sensor for one-shot profiling of human skin.

This paper describes a shimming approach useful to reduce the gradient strength of the magnetic field generated by single-sided sensors simultaneously maximizing its uniformity along the lateral directions of the magnet. In this way, the thickness of the excited sensitive volume can be increased without compromising the depth resolution of the sensor. By implementing this method on a standard U-shaped magnet, the gradient strength was reduced one order of magnitude. In the presence of a gradient of about 2 T/m, slices of 2mm could be profiled with a resolution that ranges from 25 µm at the center of the slice to 50 µm at the borders. This sensor is of particular advantage for applications, where the scanning range is of the order of the excited slice. In those cases, the full profile is measured in a single excitation experiment, eliminating the need for repositioning the excited slice across the depth range to complete the profile as occurs with standard high gradient sensors. Besides simplifying the experimental setup, the possibility to move from a point-by-point measurement to the simultaneous acquisition of the full profile led to the shortening of the experimental time. A further advantage of performing the experiment under a smaller static gradient is a reduction of the diffusion attenuation affecting the signal decay measured with a CPMG sequence, making it possible to measure the T(2) of samples with high diffusivity (comparable to the water diffusivity). The performance of the sensor in terms of resolution and sensitivity is first evaluated and compared with conventional singled-sided sensors of higher gradient strength using phantoms of known geometry and relaxation times. Then, the device is used to profile the structure of human skin in vivo. To understand the contrast between the different skin layers, the distribution of relaxation times T(2) and diffusion coefficients is spatially resolved along the depth direction.